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We narrowed to 18,759 results for: nls

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Showing: 941 - 960 of 18759 results

  3. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger
    Publication
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Mutation
    Silent change from C to A at bp 834 of insert
    Available since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45945-plasmid-map-sequence-id-116251

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  4. pCMV-MMLVgag-3xNES-ABE8e

    Plasmid
    #181751
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181751-plasmid-map-sequence-id-353935

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  6. pAC152-dual-dCas9VP64-sgExpression

    Plasmid
    #48238
    Purpose
    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    HA Tag, HA-Tag, and VP64
    Expression
    Mammalian
    Mutation
    D10A;H840A
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48238-plasmid-map-sequence-id-69545

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  7. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Publication
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112233-plasmid-map-sequence-id-213540

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  9. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51240-plasmid-map-sequence-id-410075

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  10. pTE4396

    Plasmid
    #74041
    Purpose
    Expresses human codon-optimized AsCpf1 and As crRNA.
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    As crRNA
    AsCpf1
    Use
    CRISPR
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Promoter
    CMV and human U6
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74041-plasmid-map-sequence-id-210677

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  11. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64709-plasmid-map-sequence-id-322473

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  17. pcDNA3.1-CibN-dCas9-CibN

    Plasmid
    #60553
    Purpose
    Expresses CibN-dCas9-CibN in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-CibN
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    inactivating Cas9 mutations D10A and H840A
    Promoter
    CMV
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60553-plasmid-map-sequence-id-251944

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  20. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid
    #107738
    Purpose
    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.
    Depositor
    Rylan Larsen
    Has service
    AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde
    Insert
    Cre, dTomato
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107738-plasmid-map-sequence-id-207220

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Showing: 941 - 960 of 18759 results