We narrowed to 63,805 results for: cell

1. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Promoter

   CMV, SP6

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-iCreV

   Plasmid

   #140135

   Purpose

   Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iCreV
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-PD-1 (Y223F, Y248F)-mGFP

   Plasmid

   #180790

   Purpose

   Expression of human PD-1 (Y223F, Y248F) fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   PD-1 (Y223F, Y248F) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Mutation

   PD-1 (Y223F, Y248F)

   Promoter

   SFFV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEP4 E02S ET2K

   Plasmid

   #20927

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; SV40LT and Klf4

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4 and Sox2; SV40LT and Klf4 (SOX2, POU5F1, KLF4 Human)
   

   Use

   Episomal/ebna

   Available Since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-opCas12a-6xNLS

   Plasmid

   #209022

   Purpose

   Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   opCas12a-6xNLS-Flag-Myc-NeoR
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and MYC

   Expression

   Mammalian

   Mutation

   E174R/S542R

   Promoter

   EF-1a

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMV-SB100X[EF1α-copGFP]

   Plasmid

   #218323

   Purpose

   Donor plasmid of SB100X Transposon with copGFP mammalian expression cassette

   Depositor

   Haoyi Wang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   copGFP mammalian expression cassette
   

   Expression

   Mammalian

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-PDi-CRISPRn

   Plasmid

   #73500

   Purpose

   Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   Cas9
   rtTA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CAG and TRE

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY093-EFS-BCMA-Blast-WPRE

    Plasmid

    #192194

    Purpose

    Lenti-BCMA-OE-Blast

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-BCMA-OE-Blast (TNFRSF17 Synthetic)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-hSyn-PROGERIN

    Plasmid

    #140189

    Purpose

    Overexpression of Progerin with a GFP fusion protein, under human synapsin promotor

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    Progerin (LMNA Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-8XGTIIC-DsRED-DD

    Plasmid

    #115798

    Purpose

    lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

    Depositor

    Joan Massague

    Article

    Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

    Insert

    YAP responsive promoter (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    DsRED-DHFR

    Expression

    Mammalian

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Farnselation tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTT3-ecdICAM1-ST3-His

    Plasmid

    #210666

    Purpose

    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Synthetic, Human)
    

    Tags

    Histag and Spytag003

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human IFN-gamma promoter/enhancer

    Plasmid

    #17598

    Depositor

    Howard Young

    Article

    Gonsky et al J Immunol. 2000 Feb 1. 164(3):1399-407.

    Insert

    IFN gamma promoter with 1st intron enhancer (IFNG Human)
    

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    -3.6 kb to +70 of promoter plus 680 bp of IFN-gam…

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXD024 - pLKO-EF1a-GFP-P2A-FLuc-WPRE

    Plasmid

    #192204

    Purpose

    Lenti-GL(GFP-Luciferase)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Lenti-GL(GFP-Luciferase)
    

    Use

    Lentiviral; Mammalian expression

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid

    #218973

    Purpose

    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    

    Expression

    Mammalian

    Mutation

    mutant SRSF1 binding motif (AGTCTCCT)

    Promoter

    RSV LTR

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQLL-MAG[EF1α-copGFP]

    Plasmid

    #218324

    Purpose

    Donor plasmid of MAG Transposon with copGFP mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    copGFP mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQLL-MAG-5 kb

    Plasmid

    #218305

    Purpose

    Donor plasmid of MAG Transposon with 5 kb cargo gene

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    5 kb cargo gene
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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