We narrowed to 88,257 results for: MAL

1. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-hSyn-STChRger2-TS-EYFP

   Plasmid

   #129395

   Purpose

   Soma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

   Insert

   soma targeted ChRger2
   

   Use

   AAV

   Tags

   Kv2.1-TS-EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Flag‐mTim1 (Bl/6)

   Plasmid

   #49205

   Purpose

   expresses Flag tagged mouse Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (BL/6 allele) (Havcr1 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available Since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBp-FGFR2b-K660N

   Plasmid

   #45707

   Depositor

   Matthew Meyerson

   Article

   Liao et al Cancer Res. 2013 Jun 20.

   Insert

   FGFR2 IIIb (FGFR2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   K660N

   Promoter

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCMV_UdgX-EE-UdgX-nCas9-RBMX

   Plasmid

   #163565

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)

   Promoter

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-short Cre-on G/W

   Plasmid

   #86949

   Purpose

   Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. For introducing large constructs into AAV

   Depositor

   Rob Mitra, Joseph Dougherty

   Article

   Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 PA-mCit-GW

   Plasmid

   #113448

   Purpose

   ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   ProteinA-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSF11-wmiRFP2-T2A-HO1

   Plasmid

   #179485

   Purpose

   Expresses miRFP2 and HO1in worm cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   wmIRFP2-T2A-HO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   tag-168 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS_4xG1 PylT FLAG-G1 PylRS

    Plasmid

    #154768

    Purpose

    plasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. G718A_BattB(GA)_TKterm_AttB(GT)_sGFP-PTEN-IRES-mCherry-P2A-HygroR_noTerm_attP(GA)_IRES-UnaG-2A-Puro-Term

    Plasmid

    #200634

    Purpose

    Plasmid self-excising recombination plasmid for Matreyek Bxb1(GT) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330 N-FKBP-Cas9

    Plasmid

    #162727

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

    Plasmid

    #214877

    Purpose

    Lentiviral vector encoding RfxCas13d targeting LPT guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crLPT-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102892

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLJM5-mDPYD

    Plasmid

    #61614

    Purpose

    The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT

    Depositor

    David Sabatini

    Article

    Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

    Insert

    dihydropyrimidine dehydrogenase (Dpyd Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-dCas12a-A3B

    Plasmid

    #193642

    Purpose

    Expression of base editor dCas12a-A3B in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3B, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-dCas12a-A3G

    Plasmid

    #193643

    Purpose

    Expression of base editor dCas12a-A3G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-hA3G, EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEos3-Rac1

    Plasmid

    #92039

    Purpose

    Expresses mEos3-Rac1 fusion protein

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-APPL1-ΔPTB

    Plasmid

    #59768

    Purpose

    Expresses APPL1 with PTB domain deleted

    Depositor

    Donna Webb

    Article

    Broussard et al Mol Biol Cell. 2012 Apr;23(8):1486-99. d

    Insert

    APPL1 (APPL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 467-709

    Promoter

    CMV

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

20. ABE8.8-d

    Plasmid

    #136295

    Purpose

    expresses ABE8.8-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge