We narrowed to 13,211 results for: CAR

1. Venus2-Y705F/S727A-STAT3

   Plasmid

   #123177

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30-2-GPD1L

   Plasmid

   #83912

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Venus1-S727A-STAT3

   Plasmid

   #123174

   Purpose

   Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   S727A substitution

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Venus1-Y705F/S727A-STAT3

   Plasmid

   #123176

   Purpose

   Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   Double Y705F and S727A substitutions

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Venus1-Y705F-STAT3

   Plasmid

   #123172

   Purpose

   Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   Y705F substitution

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Venus1-K685R-STAT3

   Plasmid

   #123170

   Purpose

   Expresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
   

   Tags

   Venus1 (aa 1-158) for bimolecular fluorescence co…

   Expression

   Mammalian

   Mutation

   K685R substitution

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL227 NheI-Cas9-KpnI-5xFlag-FseI-TdT-NotI

   Plasmid

   #126477

   Purpose

   Expresses Cas9-5xFlag-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   Cas9
   5xFlag
   TdT (DNTT Human)
   

   Expression

   Mammalian

   Mutation

   Changed codons to reduce repetitive sequences.

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Venus2-Y705F-STAT3

   Plasmid

   #123173

   Purpose

   Expresses Y705F STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   Y705F substitution

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Venus2-S727A-STAT3

   Plasmid

   #123175

   Purpose

   Expresses S727A STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

   Insert

   STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
   

   Tags

   Venus2 (aa 158-238) for bimolecular fluorescence …

   Expression

   Mammalian

   Mutation

   S727A substitution

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Venus1-K49R-STAT3

    Plasmid

    #123166

    Purpose

    Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    K49R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Venus2-K49R-STAT3

    Plasmid

    #123167

    Purpose

    Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    K49R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Venus1-K140R-STAT3

    Plasmid

    #123168

    Purpose

    Expresses K140R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    

    Tags

    Venus1 (aa 1-158) for bimolecular fluorescence co…

    Expression

    Mammalian

    Mutation

    K140R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Venus2-K140R-STAT3

    Plasmid

    #123169

    Purpose

    Expresses K140R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    K140R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Venus2-K685R-STAT3

    Plasmid

    #123171

    Purpose

    Expresses K695R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

    Insert

    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    

    Tags

    Venus2 (aa 158-238) for bimolecular fluorescence …

    Expression

    Mammalian

    Mutation

    K685R substitution

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid

    #61576

    Purpose

    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    chromatin insulator flanked TRE-LSL-tdTomato
    

    Use

    Recombinase-mediated cassette exchange using flp …

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-Flag-CDK8-WT-IRES-mCherry

    Plasmid

    #68856

    Purpose

    Expresses Human CDK8 Wild-Type

    Depositor

    Matthew Shair

    Article

    Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

    Insert

    cyclin-dependent kinase 8 (CDK8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE-N1-RDH11

    Plasmid

    #161916

    Purpose

    Expresses untagged human RDH11. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    Retinol dehydrogenase 11 (RDH11 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227898

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227899

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge