165,385 results

1. Tag5Amyc-GSK3b CA

   Plasmid

   #16261

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Constitutively active: S9A

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFREE_Cm

   Plasmid

   #92052

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Promoter

   pRhamBAD and ptet

   Available Since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gal:mCherry-uTEV3

   Plasmid

   #135453

   Purpose

   Yeast expression of uTEV3 fused to mCherry

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   mCherry-uTEv3
   

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   S219V mutation improves stability , I138T/S153N/T…

   Promoter

   Gal

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Orf3a-mCherry

   Plasmid

   #165138

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Orf3a (ORF3a codon optimized SARS-COV-2)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH-puro/Flag PD-L1 WT

   Plasmid

   #121446

   Purpose

   expression of huamn PD-L1 wildtype in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   wildtype

   Promoter

   CMV

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. UBC plasmid

   Plasmid

   #169746

   Purpose

   Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pF CAG luc hygro

   Plasmid

   #67502

   Purpose

   Constitutive expression of firefly luciferase in mammalian cells

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   Firefly luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGH020_sgRNA_G418-GFP

   Plasmid

   #85405

   Purpose

   hu6 driven sgRNA vector with G418 and GFP selectable markers

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   G418 resistance and GFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Integrin-Beta1-N-18

   Plasmid

   #55064

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Integrin-Beta1 (ITGB1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTlpA39-Wasabi

    Plasmid

    #86116

    Purpose

    mWasabi reporter gene gated by TlpA39

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA39
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A, D135V, A217V, L236F

    Promoter

    pTlpA and pTlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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