We narrowed to 9,619 results for: Coli

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pDN-T1TCbt

Plasmid

#44514

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-T123 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Three tandem tet operators (3XtetO2) downstream o…

Promoter

PGal1-T123 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-A2-77p-Venus

Plasmid

#64856

Purpose

Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

Depositor

Sriram Neelamegham

Article

Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

Insert

VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)

Tags

7X His and Venus

Expression

Bacterial

Promoter

T7 Promoter

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDN-G1TCbt

Plasmid

#44515

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR::ymcherry

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCK669

Plasmid

#192638

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dxCas9-NG h…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-LacI-Mit1AD

Plasmid

#126722

Purpose

LacI-Mit1AD on pGAPZ_ A expression vector

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

hybrid lacI-Mit1 activation domain coding sequence

Expression

Yeast

Promoter

GAP

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1273

Plasmid

#119265

Purpose

E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

E. coli attTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-TetR-tagRFP-T

Plasmid

#103809

Purpose

'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR-tagRFP-T (CIB1, tetR E. coli, Mustard Weed)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FB003

Plasmid

#119677

Purpose

Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

PtrpC:hph:Ttub

Use

Synthetic Biology

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

Plasmid

#92225

Purpose

Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP Y35TAG

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Mutation

Residue Y35 mutated into TAG (Amber) stop codon f…

Promoter

p70a

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc E. coli, Fly)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only