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pCS2 hSmad1-MM

Plasmid

#22990

Depositor

Edward De Robertis

Article

Pera et al Genes Dev. 2003 Dec 15. 17(24):3023-8.

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S214A, S206A, S195A, S187A

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA mouse NHERF-1 (1-355)

Plasmid

#32705

Depositor

Edward Weinman

Article

Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)

Tags

none

Expression

Mammalian

Mutation

none

Promoter

cmv

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
jk56

Plasmid

#87009

Purpose

genomic Sindbis virus plasmid for expression of SYNseq components

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

Inserts

Nrx1B
mCherry

Use

Synthetic Biology; Sindbis virus genomic plasmid

Expression

Mammalian

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PfliC-GFPLVA

Plasmid

#23342

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

Insert

GFP(LVA)

Tags

GFP(LVA)

Expression

Bacterial

Available Since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pStr-KDEL_APV-SBP-DsRed2-ER-5

Plasmid

#170675

Purpose

Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Inserts

APV-SBP-DsRed2-ER-5
streptavidin-KDEL

Expression

Mammalian

Promoter

IRES and T7

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

Plasmid

#81133

Purpose

mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
VAV2 DH/PH/C1 (VAV2 Human)

Tags

mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO1_F

Plasmid

#146314

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Expression

Mammalian

Mutation

N574S

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCK013

Plasmid

#105345

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK016

Plasmid

#105348

Purpose

Bacterial translocation for proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK014

Plasmid

#105346

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Mutation

BsaI restriction sites removed

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 uGFP

Plasmid

#14557

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
pTYF-sGFAP-IRES-tdTomato

Plasmid

#166920

Purpose

Empty plasmid backbone; includes IRES-tdTomato and sGFAP promoter

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

Type

Empty backbone

Use

Lentiviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBTK1004 (pSL3)

Plasmid

#190999

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 2

Plasmid

#73522

Purpose

Encodes the second coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 2 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
MBD1v1 pcDNA3.1

Plasmid

#78140

Purpose

Mammalian expression of MBD1v1

Depositor

Heidi Schwarzenbach

Article

Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

Insert

MBD1 (MBD1 Human)

Expression

Mammalian

Mutation

v1 isoform

Promoter

CMV

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pFSynWSyt1C2AB(80-421)msGFP

Plasmid

#209014

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pWA PAS-E BAD K-GAFm

Plasmid

#39866

Depositor

Vladislav Verkhusha

Article

Filonov et al Chem Biol. 2013 Jul 23. pii: S1074-5521(

Inserts

iRFP protein PAS domain
iRFP protein GAF domain (mutated)

Tags

E coil and K coil

Expression

Bacterial

Mutation

F165Y, D232Y, W308R

Promoter

arabinose inducible and rhamnose inducible

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pSG424 Smad2 (Gal4-Smad2)

Plasmid

#14932

Depositor

Joan Massague

Article

Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

Insert

Smad2 (SMAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only