164,247 results

1. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

   Plasmid

   #218862

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

   Insert

   RSET-jGCaMP8m
   

   Use

   Tags

   6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

   Expression

   Insect

   Mutation

   A25G F286Y

   Promoter

   hsp70

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDule-Tet2.0

   Plasmid

   #85496

   Purpose

   Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044

   Insert

   Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32G L65Q F108S Q109D D158S L162N

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-CAG-empty-pgk-hph

   Plasmid

   #48753

   Purpose

   PiggyBac backbone, CAG promoter, Hygromycin selection

   Depositor

   Austin Smith

   Article

   Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

   Type

   Empty backbone

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pET21 MBPx-SpyCatcher

   Plasmid

   #72327

   Purpose

   Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET21 MBPx-SpyCatcher
   

   Use

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Mutation

   MBP contains A312V, I317V mutations and 172, 173,…

   Promoter

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223_SRC_WT

   Plasmid

   #82165

   Purpose

   Gateway Donor vector containing SRC , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SRC (SRC Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAVU6+27-F30-2xdBroccoli

   Plasmid

   #66842

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. hE-cadherin/Δp120

   Plasmid

   #45770

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Δp120 (CDH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 762-764 converted from EED to AAA to …

   Promoter

   CMV

   Available Since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pMaCTag-P07

   Plasmid

   #124788

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeonGreen
   

   Use

   Pcr template

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTight-hMYT1L-N174

   Plasmid

   #31877

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   myelin transcription factor 1-like (MYT1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV.tetO.Ascl1.U6.shRest1.U6.shRest2

    Plasmid

    #234846

    Purpose

    3rd generation lentiviral vector, expresses Ascl1 under control of TetON promoter and two shREST sequences under control of U6 promoters

    Depositor

    Malin Parmar

    Article

    Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP-NOP58-C1

    Plasmid

    #134537

    Purpose

    Expresses YFP-NOP58 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    NOP58 (NOP58 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe JUND-HA neo

    Plasmid

    #58489

    Purpose

    mammalian expression of human JUND

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JUND (JUN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_FOXA2_P2A_Hygro_Barcode

    Plasmid

    #120439

    Purpose

    Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA2 (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

    Plasmid

    #180263

    Purpose

    Lentiviral expression vector for dCas9-VP64 in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

    Plasmid

    #101282

    Purpose

    Lentiviral expression of shRNA and mouse E-cadherin -mCherry

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-cadherin
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-opto-mRipK3

    Plasmid

    #208783

    Purpose

    Inducibly expresses mouse RipK3 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mRipK3 Cry2 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Cry2 and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL5.0 E-cadherin shRNA/Ecad-tdTomato

    Plasmid

    #101278

    Purpose

    Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-cadherin
    

    Use

    Lentiviral

    Tags

    tdtomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-ALK5 K232R

    Plasmid

    #80878

    Purpose

    mammalian expression of ALK5 K232R

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K232R (kinase dead)

    Promoter

    CMV

    Available Since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

    Plasmid

    #207491

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.4_1G11F2-3 light chain

    Plasmid

    #198254

    Purpose

    recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

    Depositor

    Aymelt Itzen

    Article

    Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

    Insert

    light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)
    

    Use

    Tags

    IL10 signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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