We narrowed to 5,621 results for: pcas

1. pCAG-1700029I15Rik-3×FLAG

   Plasmid

   #199689

   Purpose

   Expression vector of mouse 1700029I15Rik. 3XFLAG tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Lu et al Proc Natl Acad Sci U S A. 2023 Feb 21;12

   Insert

   1700029I15Rik (1700029I15Rik Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG.Myc-SARS2_3CL(C145A)

   Plasmid

   #200121

   Purpose

   Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL C145A in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   CAG

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG1.1-Tsks/1D4

   Plasmid

   #199623

   Purpose

   Expression vector of mouse TSKS (variant X6). 1D4 tag at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Shimada et al Proc Natl Acad Sci U S A. 2023 Mar 14;12

   Insert

   Tsks (variant X6) (Tsks Mouse)
   

   Use

   Tags

   1D4

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas-mut4/1_2_VB

   Plasmid

   #186444

   Purpose

   Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas4/1 and Cas2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas4 domain (K81A)

   Promoter

   T7

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI

   Plasmid

   #195504

   Purpose

   Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAS-mak10 gRNA

   Plasmid

   #160385

   Purpose

   Expresses guide RNA for targeting MAK10 in yeast

   Depositor

   John McCusker

   Insert

   mak10 gRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RNA pol III promoter (tRNA-Tyr)

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXD70LacZ8.1-Pcadh-LacZ

   Plasmid

   #191624

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-3xHAtag-SP-CLSTN2

   Plasmid

   #191694

   Purpose

   For expression coding sequencing of CLSTN2

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   signal peptide, 3xHAtag and full length coding sequence of CLSTN2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-SP-3xHAtag-PTPRO

   Plasmid

   #191695

   Purpose

   For expression coding sequencing of PTPRO

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   signal peptide, full length coding sequence of PTPRO and 3xHAtag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-ATG13 (1-197)

    Plasmid

    #188074

    Purpose

    To make ATG101/ATG13 HORMA complex for expression in mammalian cells

    Depositor

    James Hurley

    Article

    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

    Insert

    ATG13 (ATG13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon-optimized ATG13 (1-197)

    Promoter

    CMV

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-mTurbo-mito

    Plasmid

    #187177

    Purpose

    Express mitochondira-localized miniTurbo under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

    Insert

    mtActA fused with miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-H2B-mTurbo

    Plasmid

    #187178

    Purpose

    Express histone H2B tagged with miniTurbo under the control of CAG promoter

    Depositor

    Isei Tanida

    Article

    Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

    Insert

    histone H2B fused with miniTurbo (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLPC-FLAG.Mm.EPC1.delta.EPcA

    Plasmid

    #184655

    Purpose

    Retroviral expression of mouse EPC1 delta EPcA

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    epc1 delta EPcA

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPC-Flag.Mm.Epc1-EPcA

    Plasmid

    #184657

    Purpose

    Retroviral expression of mouse Epc1 EPcA

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Epc1 (Epc1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Epc1-EPcA

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJCC_103 SpCas9 T1337C

    Plasmid

    #179525

    Purpose

    For bacterial expression of SpCas9 T1337C (for DNA cross-linking) with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

    Insert

    SpCas9 T1337C
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    changed threonine 1337 to cysteine

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA528 CHMP1B 4-199

    Plasmid

    #115325

    Purpose

    Expresses residues 4-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-23

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA528 CHMP1B 1-199

    Plasmid

    #115326

    Purpose

    Expresses residues 1-199 of CHMP1B from a His-SUMO bacterial expression vector. Internal ID: WISP 18-24

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG1.1-Armc12/FLAG

    Plasmid

    #180494

    Purpose

    Expression vector of mouse armadillo repeat containing 12 (Armc12) tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

    Depositor

    Masahito Ikawa

    Article

    Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

    Insert

    armadillo repeat containing 12 (Armc12 Mouse)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCASCADE-gltA1-gltA2

    Plasmid

    #71348

    Purpose

    gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction

    Depositor

    Michael Lynch

    Article

    Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

    Insert

    gltA1-gltA2 guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCASCADE-udhA-xylA

    Plasmid

    #158612

    Purpose

    Low phosphate inducible gRNA array to silence the xylA and udhA promoters

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    udhA-xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCASCADE-gltA2-xylA

    Plasmid

    #158613

    Purpose

    Low phosphate inducible gRNA array to silence the xylA and gltA2 promoters

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    gltA2-xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCASCADE-zwf-xylA

    Plasmid

    #158614

    Purpose

    Low phosphate inducible gRNA array to silence the xylA and zwf promoters

    Depositor

    Michael Lynch

    Article

    Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

    Insert

    zwf-xylA guide array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E . coli ugpB gene promoter, low phosphate induct…

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCAG-mSpata33 (I81A)-PA

    Plasmid

    #179131

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Changed Isoleucine 81 to Alanine

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-mSpata33 (P79A)-PA

    Plasmid

    #179130

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Changed Proline 79 to Alanine

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-mSpata33 (I83A)-PA

    Plasmid

    #179132

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I83A mutation is introduced in Spata33.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Changed Isoleucine 83 to Alanine

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDSARN-vasa-eSpCas9sv40

    Plasmid

    #173670

    Purpose

    eSpCas9 expression vector for Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Insert

    eSpCas9 with Anopheles vasa promoter and SV40 terminator
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    mutations in Cas9 reducing off-target activity (S…

    Promoter

    vasa

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330_ACTB-e1 sgRNA / hSpCas9

    Plasmid

    #172830

    Purpose

    Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid

    #172831

    Purpose

    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pX330_Camk2a sgRNA / hSpCas9

    Plasmid

    #172839

    Purpose

    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAFNF-LifeAct-EGFP

    Plasmid

    #163608

    Purpose

    Expresses LifeAct fused to EGFP Flp-dependently in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    LifeAct
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge