Addgene

We narrowed to 4,364 results for: DUR

Showing: 1921 - 1960 of 4364 results

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myc-hRAD18 d6BD

Plasmid

#68832

Purpose

Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only
pmiRGLO-BAX-201

Plasmid

#117332

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR BAX-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon3_2_Lb

Plasmid

#155054

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLCHKO_HPRT1_exon2_1_Lb

Plasmid

#155051

Purpose

Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

(hg)RNA for deletion of HPRT1 exon 2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-ATF5-201

Plasmid

#117340

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR ATF5-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p1.2-GS

Plasmid

#162770

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Topo_SpCas9.tracr_LbCas12a.DR

Plasmid

#155049

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_PSMB2_sgRNA_1

Plasmid

#74184

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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PB-CAG-Haunt-EF1a-DsRed

Plasmid

#92162

Purpose

Haunt in trans overexpression plasmid

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt (Halr1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD53

Plasmid

#172457

Purpose

Expression of Tumor Protein D52 like 1 (TPD53/TPD52L1) with N-terminal mCherry-FKBP tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD53 (TPD52L1 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-CNPsgRNA

Plasmid

#126610

Purpose

Expresses CNP sgRNA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

CNP sgRNA (Cnp Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-MIRΔ

Plasmid

#160995

Purpose

Express mouse p150 protein with MIR deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

MIR (HP1-interacting domain) deletion

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150-PIP2Δ

Plasmid

#160994

Purpose

Express mouse p150 protein with PIP2 deletion mutation

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

PIP2 motif deletion

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S708/710A

Plasmid

#70153

Purpose

mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S708A, S710A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Promoter

Available since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
pmiRGLO-LFNG-203

Plasmid

#117351

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR LFNG-203

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-MKLP1 S708A

Plasmid

#70146

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S708A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S708A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Topo_SpCas9.tracr_AsCas12a.DR

Plasmid

#155050

Purpose

TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

Depositor

Benjamin Blencowe, Jason Moffat

Article

Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

Insert

SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLCKO_PSMB2_sgRNA_5

Plasmid

#74185

Purpose

lentiviral vector expressing sgRNA targeting PSMB2

Depositor

Jason Moffat

Article

Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

Insert

PSMB2 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 Promoter

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-CD164-201

Plasmid

#117329

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR CD164-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO-TMEM63C-201

Plasmid

#117372

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

TMEM63C-2013' UTR

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pmiRGLO-TNFRSF10B

Plasmid

#117373

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR TNFRSF10B

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO-KIAA2013-201

Plasmid

#117348

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR KIAA2013-201

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO-FAM102A-203

Plasmid

#117344

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR FAM102A-203

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRGLO-GRM4-202

Plasmid

#117347

Purpose

Dual luciferase assay for miRNA-targeted UTR

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

3' UTR GRM4-202

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Murine PGK-1

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN GBRP

Plasmid

#89293

Purpose

For expression of the mammalian Atg8 protein GABARAP without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-Flag-MyoD(QQQ)-E12

Plasmid

#26003

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Expression

Mutation

Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

Promoter

Available since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
Alt AlphaPS3

Plasmid

#14070

Depositor

Richard Hynes

Article

Stark et al Development. 1997 Nov . 124(22):4583-94.

Insert

Drosphila Alt Alpha PS3 Integrin (scb Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV-miR30-Vector

Plasmid

#171194

Purpose

Retroviral vector for U6 promoter driven expression empty miR30 based shRNA (to be used in conjunction with Phoenix packaging cells).

Depositor

Masashi Narita

Article

Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

Insert

shRNA empty vector

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)

Plasmid

#214889

Purpose

Expresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB27 (pmyo-3::GtACR2::mCerulean::βHK::Chrimson)

Plasmid

#214890

Purpose

Expresses the optogenetic tool BiPOLES in BWMs of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB17 (punc-17::QuasAr)

Plasmid

#214888

Purpose

Expresses the voltage sensor QuasAr2 in cholinergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

QuasAr2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)

Plasmid

#214891

Purpose

Expresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.

Depositor

Alexander Gottschalk

Article

Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

Insert

GtACR2_mCerulean_bHK_Chrimson

Use

Tags

mCerulean

Expression

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mCHERRY

Plasmid

#224298

Purpose

Expresses mCherry under EF1a promoter (CTRL vector)

Depositor

Nikhil Joshi

Article

Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

Insert

mCherry

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

Plasmid

#229995

Purpose

PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

Depositor

Christian Mayer

Article

Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

Type

Empty backbone

Use

CRISPR

Tags

NotI site for NEBuilder HiFi DNA Assembly with ss…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LC3B-TdLanYFP

Plasmid

#228565

Purpose

Encodes an acceptor-only, TdLanYFP-tagged version of the LC3B biosensor under the control of the CMV promoter

Depositor

Giulia Bertolin

Article

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Tags

TdLanYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1921 - 1960 of 4364 results

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