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Plasmid#114667PurposeExpresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationchanged serine 122 to aspartic acidPromoterATG9Available sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis (1-440)
Plasmid#60391PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-HA-SrtAstrep (pKS78)
Plasmid#50029PurposeGalactose-inducible expression of cytosolic S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
UseTagsHAExpressionYeastMutationPromoterpGALAvailable sinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSF-KpDcr1deltaC(E224Q, GiVL-1)
Plasmid#32033DepositorInsertDCR1(15-355)
UseTagsHis6-SUMOExpressionBacterialMutationE224Q and H123-N143 replaced by P642-Y645 of GiDi…PromoterT7Available sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE intramol-10u
Plasmid#13550DepositorInsertFLIP-E intramol A207R (gltI E. coli)
UseTagsEYFPExpressionBacterialMutationA207R, ECFP insert in binding proteinPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
Sc [Pol31 + Pol32]-pRS403/GAL
Plasmid#239199PurposeOvererxpress Sc Pol31 & Pol32 when integrated into yeast (S. cer)DepositorUseTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Sc Pol3-FLAG(Ec)-pRSF405/GAL
Plasmid#239198PurposeOverexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)DepositorInsertPol3 (POL3 Budding Yeast)
UseTags3X FLAGExpressionYeastMutationE. coli codon bias (synthetic gene), but same ami…PromoterAvailable sinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3E's)-pRSFDuet
Plasmid#238344Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96E; R103E; R105E) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96E; R103E; R105EPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3A's)-pRSFDuet
Plasmid#238343Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96A; R103A; R105A) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96A; R103A; R105APromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec FkP-dnaN(3S's)-pRSFDuet
Plasmid#238345Purposeover-expresses PreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta mutant in E. coliDepositorInsertPreScission Protease-cleaveable N-tagged FLAG-kinase E. coli beta (R96S; R103S; R105S) (dnaN E. coli)
UseTags3X FLAG+PKA kinase motif+PreScission Protease cle…ExpressionBacterialMutationR96S; R103S; R105SPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-SEC61-FAPoptim
Plasmid#221126PurposeFAP-tagged marker for the ER (FAP optimized for yeast expression)DepositorInsertSEC61-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC63-FAPoptim
Plasmid#221116PurposeFAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertFAPoptim-SEC63
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC61-FAPoptim
Plasmid#221115PurposeFAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertSEC61-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-STE3-GFP
Plasmid#221113PurposeGFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)DepositorInsertSTE3-GFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 817 Truncation
Plasmid#234126PurposeSyf1 PCR truncation starting at 817 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 634 Truncation
Plasmid#234116PurposeSyf1 PCR truncation starting at 634 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 778 Truncation
Plasmid#234115PurposeSyf1 PCR truncation starting at 778 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-ANP1-FAPoptim
Plasmid#221120PurposeFAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)DepositorInsertANP1-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-ERG6-FAPoptim
Plasmid#221121PurposeFAP-tagged marker for lipid droplets (FAP optimized for yeast expression)DepositorInsertERG6-FAPoptim
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only