We narrowed to 25,334 results for: Spr

Showing: 9861 - 9880 of 25334 results

Results per page:

Sort results by:

pJE53

Plasmid

#194153

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pQdC12a

Plasmid

#191356

Purpose

Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Inserts

PGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR

Expression

Bacterial

Promoter

PGus2A-TetO2/1 and miniPthl

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
gh123

Plasmid

#106790

Purpose

expression of gRNA targeting ST8SIA3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA3 (ST8SIA3 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9 fused to nanobody (anti-beta catenin)

Plasmid

#186420

Purpose

Expression of dCas9 with C-terminal nanobody fusion recognizing beta catenin tag

Depositor

Stephen Elledge

Article

Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

Insert

dCas9-nanobody fusion (anti-beta catenin)

Use

CRISPR

Tags

6His

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Nanog-2A-mCherry

Plasmid

#48680

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Nanog (Nanog Mouse)

Use

CRISPR

Tags

Cherry

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LCV2 control

Plasmid

#217443

Purpose

Lentiviral vector expressing Cas9 without a targeting sgRNA

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pY2ShHELIX_sgRNA_entry (CJT30)

Plasmid

#181781

Purpose

Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k

Expression

Bacterial

Mutation

Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

Promoter

Lac and J23119

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDIT_Cas9-MS2-BB_BbsI

Plasmid

#164802

Purpose

REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1183

Plasmid

#119254

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
LbCas12a-nuCPE

Plasmid

#213748

Purpose

For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-LbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R in LbCas12a

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEX-SaCas9-U6-sgSlc18a2

Plasmid

#124849

Purpose

Mutagenesis of Slc18a2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6_(GLuc)_INT[3xMS2_SL]

Plasmid

#68426

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three MS2 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-MMLVgag-D3A-3L-dCas9

Plasmid

#240530

Purpose

Expresses MMLVgag–DNMT3A-3L-dCas9 for producing RENDER-DNMT3A-3L-dCas9

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

MMLVgag-D3A-3L-dCas9

Tags

FLAG, HA

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLoxP-DHFR-mCherry

Plasmid

#70147

Purpose

Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion

Depositor

David Sibley

Article

Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/

Insert

a selectable dihydrofolate reductase-thymidylate synthase marker

Use

Expression in toxoplasma gondii

Tags

mCherry

Promoter

DHFR 5' UTR

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR1276

Plasmid

#111115

Purpose

Expresses RAD51C_+_56769980.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

RAD51C gRNA (RAD51C Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pREDIT_Cas9n-MS2-BB_BbsI

Plasmid

#164804

Purpose

REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge