We narrowed to 11,459 results for: 110

1. pMOD_C2515

   Plasmid

   #91083

   Purpose

   Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   AtU6

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)- e18 LOF

   Plasmid

   #131729

   Purpose

   Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD279.8

   Plasmid

   #173171

   Purpose

   Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

   Depositor

   Jeffrey Tabor

   Article

   Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

   Inserts

   SO_4388(REC)-PsdR(DBD) 137
   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23108 and PpsdA110

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nrp2.3-AP-His

   Plasmid

   #71974

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.3 (Nrp2 Mouse)
   

   Use

   Tags

   AP-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_C2518

   Plasmid

   #91087

   Purpose

   Module C, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   TaU6

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW648 - pREV>>

   Plasmid

   #115996

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Use

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-Lifeact-EGFP-3xHA (JDW 1322)

   Plasmid

   #224494

   Purpose

   Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Liefact-EGFP-3xHA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSW505 - pOp4:3xGFP-NLS

   Plasmid

   #116005

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR
   

   Use

   Tags

   linker-NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIG-VP16–RAR-alpha

   Plasmid

   #80434

   Purpose

   Expression of constitutively active human RAR-alpha in chick embryos

   Depositor

   Aixa Morales

   Article

   Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

   Insert

   Retinoic acid receptor alpha (RARA Human)
   

   Use

   Tags

   EGFP and VP16

   Expression

   Mammalian

   Mutation

   Promoter

   beta-actin promoter

   Available since

   Jan. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pAPM-D4 miR30-FAM208A ts1

    Plasmid

    #115871

    Purpose

    FAM208a knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    FAM208a shRNA (C3orf63 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF1472

    Plasmid

    #141825

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TOX2 (TOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TMEM16F-M522K-peGFP-N1

    Plasmid

    #228579

    Purpose

    Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M522K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMEM16F-T526K-peGFP-N1

    Plasmid

    #228580

    Purpose

    Expresses mouse TMEM16F-T526K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T526K plus a 3 amino acid N-terminal truncation (…

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsDCP2-E148Q_U

    Plasmid

    #147650

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. p5E-TetO8x-CMVmin (JDW 1087)

    Plasmid

    #224475

    Purpose

    Gateway compatible 5' entry clone containing 8 tet operons (TREs) upstream of a minimal CMV promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    TetO(8x)-CMV promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCALPS-mAgo2-EGFP

    Plasmid

    #209935

    Purpose

    Lentiviral expression of mouse Argonaute-2 in mammalian cells

    Depositor

    Phillip Zamore

    Article

    Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRL-TK 3'FBXW7 UTR

    Plasmid

    #26649

    Depositor

    Alex Minella

    Article

    Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

    Insert

    FBXW7 (FBXW7 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Contains only the 3'UTR of FBXW7, nucleotide…

    Promoter

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)

    Plasmid

    #129667

    Purpose

    A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-MS2-HA-HsNot4_AA

    Plasmid

    #148255

    Purpose

    Mammalian Expression of HsNot4

    Depositor

    Elisa Izaurralde

    Article

    Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

    Insert

    HsNot4 (CNOT4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation compared to the sequence give…

    Promoter

    Available since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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