We narrowed to 13,211 results for: CAR

1. pLKO.1-TRC.mKO2_shGLS

   Plasmid

   #110319

   Purpose

   shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene and express monomeric Kusabira-Orange2

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Insert

   GLS Glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3 ATP5O(TISU)-FF

   Plasmid

   #85489

   Purpose

   Firefly luciferase under the control of ATP5O TISU

   Depositor

   Ivan Topisirovic

   Article

   Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

   Insert

   Full TISU element of ATP5O (ATP5PO Human)
   

   Use

   Luciferase

   Tags

   Firefly luciferase

   Expression

   Mammalian

   Mutation

   2nd and 3rd codon of luciferase were modified fro…

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

   Plasmid

   #128588

   Purpose

   AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   ChrimsonR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   EF1α (1.1 kb short version)

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_V5.AU1.FLAG_NGFR

   Plasmid

   #158240

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5.AU1.FLAG

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CRY2low-tdTomato-Raf1

   Plasmid

   #104176

   Purpose

   Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato and Raf1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2low-tdTomato-Raf1 (CRY2, RAF1 Human, Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   CRY2 aa 1-491 truncation, R489E A491D

   Promoter

   CMV

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO_NWS.V5.VSVg_NGFR

   Plasmid

   #158246

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.V5.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO_V5.FLAG.VSVg_NGFR

   Plasmid

   #158247

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   V5.FLAG.VSVg

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO_NWS.V5.FLAG_NGFR

   Plasmid

   #158251

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   NWS.V5.FLAG

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available Since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

   Plasmid

   #117139

   Purpose

   Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP

   Depositor

   Kosuke Yusa

   Article

   Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

   Insert

   HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   modified BFP, modified Dmap1 CDS

   Promoter

   human U6 promoter, human EF1a promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-PHB-6

    Plasmid

    #122232

    Purpose

    Expresses shRNA targeting the coding sequence of human PHB

    Depositor

    Alex Kentsis

    Article

    Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

    Insert

    shRNA targeting PHB (see partial depositor seq) (PHB1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO_S.V5.HA_NGFR

    Plasmid

    #158339

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.V5.HA

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO_Ollas.AU1.C_NGFR

    Plasmid

    #158314

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Ollas.AU1.C

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO_HA.AU1.V5_NGFR

    Plasmid

    #158235

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.AU1.V5

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL4.23 C3_9

    Plasmid

    #60295

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    NKX6-1 enhancer (NKX6-1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pENTR1A_mmFbxw7a (closed)

    Plasmid

    #160101

    Purpose

    Expresses murine Fbxw7 alpha in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Gateway entry vector

    Expression

    Mammalian

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMS1401

    Plasmid

    #29198

    Purpose

    High copy homologous recombination plasmid for gene targeting at the mouse Hprt locus containing Ple67 (FEV MiniPromoter) driving an EGFP reporter

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    Ple67 (FEV Human)
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Available Since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR

    Plasmid

    #113162

    Purpose

    A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    SpCas9
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid

    #114071

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R and S542R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_TH1477_2xUGI_3xHA

    Plasmid

    #136659

    Purpose

    Expresses St1BE4max-TH1477 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:TH1477 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRY2low-mCherry-Raf1

    Plasmid

    #104066

    Purpose

    Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1

    Depositor

    Bianxiao Cui

    Article

    Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

    Insert

    CRY2low-mCherry-Raf1 (CRY2, RAF1 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    CRY2 aa 1-491 truncation, R489E A491D

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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