We narrowed to 24,993 results for: Nes

Showing: 9901 - 9920 of 24993 results

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EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Talin-18

Plasmid

#55447

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Talin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-GW

Plasmid

#63780

Purpose

yeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Article

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only
pMB62

Plasmid

#47944

Purpose

Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pMC-J6-dCas9-24XGP41-J9

Plasmid

#202006

Purpose

MoClo level 0 dCas9-24XGP41 coding sequence module

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Insert

dCas9-24XGP41

Use

Synthetic Biology; Moclo cloning vector

Tags

GS linkers

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL70004

Plasmid

#50334

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-22

Plasmid

#64229

Purpose

Expresses miR-22 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR22 (MIR22, MIR22HG Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-ACTLg

Plasmid

#61195

Purpose

Fluorescent reporter for F-actin LifeAct-eGFP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-eGFP

Tags

eGFP

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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LI F-G Spec

Plasmid

#104521

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Spectinomycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KSTbx2.8.2

Plasmid

#42626

Depositor

Virginia Papaioannou

Article

Chapman et al Dev Dyn. 1996 Aug;206(4):379-90.

Insert

Tbx2 (Tbx2 Mouse)

Expression

Bacterial

Promoter

T3

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
pHX_gRNA_hPAX7_6-9S

Plasmid

#160453

Purpose

sgRNA targeting human PAX7 3' UTR with 17bp spacer

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Insert

gRNA_hPAX7_6-9S

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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p1.2-Hygro

Plasmid

#162741

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-ciBAR1

Plasmid

#200440

Purpose

Expresses Flag-tagged human ciBAR1 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

Insert

ciBAR1

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-ciBAR2

Plasmid

#200441

Purpose

Expresses Flag-tagged human ciBAR2 in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

Insert

ciBAR2

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad3 (EPSM)

Plasmid

#14963

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (EPSM) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Mutation

T178V, S203A, S207A, S212A

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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MSX2-pET

Plasmid

#21025

Depositor

Corey Largman

Article

Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

Insert

MSX2 (Msx2 Mouse)

Tags

HIS and T7

Expression

Bacterial

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-KG-GST-YAP-S127A

Plasmid

#33053

Depositor

Kunliang Guan

Article

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

YAP (YAP1 Human)

Tags

GST

Expression

Mammalian

Mutation

S127A

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only