We narrowed to 11,455 results for: Vars;

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pAc5.1B-EGFP-DmGW182

Plasmid

#22419

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

Plasmid

#45578

Purpose

Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

coY2 Ani

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-GR-AKAR3

Plasmid

#173016

Purpose

Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

Depositor

Jin Zhang

Article

Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

Insert

Lyn-GR-AKAR3

Tags

EGFP, N-terminal targeting sequence from Lyn kina…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pYC119

Plasmid

#63916

Purpose

Recipient overexpressing vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Promoter

TEF1 (Saccharomyces cerevisiae)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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scribble cDNA

Plasmid

#42064

Depositor

Norbert Perrimon

Article

Bilder et al Nature. 2000 Feb 10;403(6770):676-80.

Insert

scribble (scrib Fly)

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
AAV-hRedOpsin-TGFB3

Plasmid

#164429

Purpose

AAV plasmid expressing TGFB3 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

Insert

Tgfb3 (Tgfb3 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGW182

Plasmid

#22406

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pEF-HA-Net1

Plasmid

#28264

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Tags

2x HA

Expression

Mammalian

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
G273D HA-dynamin 1 pUHD10-3

Plasmid

#34689

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1 Human)

Tags

HA (variant)

Expression

Mammalian

Mutation

G273D

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
Split-RESCUE-C

Plasmid

#170154

Purpose

AAV vector carrying the RESCUE-DDC-λN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

RESCUE-DDC-λN-NES

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pJH579

Plasmid

#172163

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD JM10.1); AraC

Expression

Bacterial

Mutation

Sumo(S6L) PylB(N61S, E84K, E122K, E175K, E178K, G…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SEPT11 sh1 shRNA

Plasmid

#122020

Purpose

Knock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 shRNA (Sept11 Rat)

Use

RNAi

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP2-V5His6

Plasmid

#22410

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

decapping protein 2, variant A (DCP2 Fly)

Use

Drosophila expression

Tags

EGFP and V5-His6

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJAZ10::LUC

Plasmid

#141172

Purpose

Luminescent reporter for jasmonic acid signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtJAZ10 promoter

Use

Luciferase

Expression

Plant

Promoter

AtJAZ10, AT5G13220

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gα13(EE)-YFP

Plasmid

#171374

Purpose

Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Ga13(EE)-YFP

Tags

Glu-Glu

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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px330 EQR

Plasmid

#101733

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmXRN1_A

Plasmid

#145874

Purpose

Insect Expression of DmXRN1

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmXRN1 (pcm Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-DAAT-V33G/T242G

Plasmid

#159278

Purpose

Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

Depositor

Roberto Chica

Article

Parmeggiani et al ACS Catalysis 9, 3482–3486.

Insert

Engineered d-alanine aminotransferase

Tags

6x His tag

Expression

Bacterial

Mutation

Valine 33 and threonine 242 have been mutated to …

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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P315-FBP_10

Plasmid

#139701

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-SELF

Plasmid

#158770

Purpose

Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.

Depositor

Edwin Chapman

Article

Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

Insert

Synaptophysin 1-NS3/4a (Syt1 Mouse)

Use

Lentiviral

Mutation

Flexible linkers, ADVVCC/QMSY cleavage site

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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