We narrowed to 10,580 results for: ada

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

Plasmid

#219823

Purpose

EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

Depositor

Philipp Maass

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core promoter

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pKB01_mCherry

Plasmid

#69104

Purpose

Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

Depositor

Jan-Willem Veening

Article

Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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MT363

Plasmid

#65858

Purpose

The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

nahG

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pT2/CMV-Luc/PGK-SB100X

Plasmid

#192869

Purpose

Carries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.

Depositor

Maria Castro, David Largaespada

Article

Calinescu et al J Vis Exp. 2015 Feb 22;(96). doi: 10.379

Inserts

luc
SB100X

Expression

Mammalian

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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HT028_FCMV_CheRiff_EGFP

Plasmid

#208605

Purpose

Expressing CheRiff_EGFP under CMV promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

CheRiff_EGFP

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-C7orf59

Plasmid

#42332

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

C7orf59 (LAMTOR4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pOsTIR1w/oGFP

Plasmid

#102883

Purpose

Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

OsTIR1

Tags

3x myc

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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iSpyMac

Plasmid

#155014

Purpose

Expresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Commun. 2020 May 18;11(1):2474. doi:

Insert

iSpyMac

Expression

Mammalian

Mutation

PID grafted onto SpyCas9 N-terminal domain, R221K…

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAS9i

Plasmid

#141249

Purpose

Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

Depositor

Adam Bertl

Article

Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Promoter

GAL1 (Cas9), pSNR52 (gRNA)

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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TL026_AAV_hSyn:SEP-GluA1

Plasmid

#192519

Purpose

Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

SEP-GluA1

Use

AAV and Cre/Lox

Tags

super ecliptic phluorin

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-BICDN-CIBN

Plasmid

#102254

Purpose

Expresses fusion of dynein adapter BICDN (1-594) with GFP and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

GFP-BICDN-CIB1 (CIB1 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS013E: Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163073

Purpose

Entry vector for: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFEndoNA

Plasmid

#174724

Purpose

Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

Depositor

Jukka Finne

Article

Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

Insert

GFP-endoNA fusion protein

Tags

Histag

Expression

Bacterial

Promoter

T5

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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CP1028-ABE8e

Plasmid

#138492

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-nSpCas9 (CP1028)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRIF Flag

Plasmid

#41550

Depositor

Kate Fitzgerald, Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

Trif (TICAM1 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 26, 2013

Availability

Academic Institutions and Nonprofits only
pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al Development. 2025 May 1;152(9):dev204506

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SpdCas9-VP64

Plasmid

#115794

Purpose

Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

VP64

Use

AAV

Tags

3XFLAG and dCas9-VP64

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLS-CMV-mP-Rluc

Plasmid

#213507

Purpose

CMV-driven Renilla luciferase expression vector for luciferase lentivirus-based reporter assay

Depositor

Nadav Ahituv

Article

Deng et al Science. 2024 May 24;384(6698):eadh0559.

Insert

Luciferase

Use

Lentiviral

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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