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pCiGSΔΨ

Plasmid

#108312

Purpose

Foamy virus helper plasmid expressing Gag, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Josephson et al Hum Gene Ther. 2004 Jan;15(1):87-92.

Insert

gag

Expression

Mammalian

Mutation

pCiGSΔΨ is a newer version of the pCiGS Gag expre…

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F2

Plasmid

#236432

Purpose

transient overexpression of E2F2 in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F2 (E2F2 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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hDAGLa-V5

Plasmid

#87676

Purpose

Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Tags

V5 tag

Expression

Mammalian

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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PGK-SMASH-NTERM-GFP

Plasmid

#185771

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-IKZF3-NTERM-GFP

Plasmid

#185764

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-IKZF3-NTERM-GFP

Plasmid

#185774

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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HDM_omicron_B11529_IDTDNA

Plasmid

#204142

Purpose

Expression plasmid for SARS-CoV-2 BA.1 variant spike

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

SARS-CoV-2 BA.1 variant spike (S SARS-CoV-2)

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-NTERM-GFP

Plasmid

#185761

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pmGFP11C-Tau

Plasmid

#71434

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Tags

GFP (213-228)

Expression

Mammalian

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only
pmGFP10C-Tau

Plasmid

#71433

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71434

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Tags

GFP (1-212)

Expression

Mammalian

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
PGK-IKZF3-CTERM-GFP

Plasmid

#185779

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, IKZF3 minidegron

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-SMASH-CTERM-GFP

Plasmid

#185776

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

PGK

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168

Plasmid

#133976

Purpose

mammalian expression vector of Flag tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Tubulin-mGreenLantern

Plasmid

#164460

Purpose

Localization of mGreenLantern fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Tubulin

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-Puro-H2BC11

Plasmid

#227332

Purpose

Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Plenti-ARE-HSVTK-luc

Plasmid

#161789

Purpose

Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

Depositor

Hans-Guido Wendel

Article

Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

Inserts

Luciferase-T2A-HSV-TK
mCherry P2A blasticidin

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a and Murine Gclc ARE

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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1477 pcDNA3 flag HA Akt1

Plasmid

#9021

Depositor

William Sellers

Article

Hsieh et al Nucleic Acids Res. 2004 . 32(3):893-901

Insert

Akt1 (AKT1 Human)

Tags

HA and flag

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pMXs-GATA2

Plasmid

#131602

Purpose

expression of human GATA2 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

GATA2 (GATA2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TOMM20

Plasmid

#207789

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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