We narrowed to 17,163 results for: EMB

Showing: 9941 - 9960 of 17163 results

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pLacIBD::SDRX (GB0673)

Plasmid

#68183

Purpose

Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LacI Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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mCit-Snap25b D179K A199R

Plasmid

#53259

Purpose

SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Mutation

D179K and A199R

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only
pTV-Met-Eif4a1-tdiRFP670-PEST

Plasmid

#122371

Purpose

Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST-2A with homology arms for Eif4a1

Use

Mouse Targeting

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-L

Plasmid

#122346

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc- mCE(Del 25C)

Plasmid

#82472

Purpose

Expresses myc tag and mRNA capping enzyme without 25 amino acid from C-terminal

Depositor

Daniel Schoenberg

Article

Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

Insert

myc-mCE-C terminal deleted (Rngtt Mouse)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pPICZ:hTRAAK(G124I)

Plasmid

#130672

Purpose

P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

Insert

KCNK4 (KCNK4 Human)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

N104Q, N108Q, G124I

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4DdeltaC

Plasmid

#51602

Purpose

expresses Sema4D lacking C terminus

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Tags

myc

Expression

Mammalian

Mutation

Last 69 amino acids of Sema4D C-terminus are dele…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
6xHis-TEV-SMO-1(GG)

Plasmid

#87054

Purpose

Expresses 6xHis-tagged smo-1(GG), with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

smo-1 (smo-1 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The last aa was removed to render processed SMO-1…

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJ02B2Gm:Rm_AF

Plasmid

#66061

Purpose

MoClo Device: FACS Standard Color Controls - High GFP (first unit) & RFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E:J23102:B:BCD2:C:E1010m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm:Gm_AF

Plasmid

#66062

Purpose

MoClo Device: FACS Standard Color Controls - High RFP (first unit) & GFP (second unit) expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E:J23102:B:BCD2:C:E0040m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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WPXL SOX10 gRNA

Plasmid

#101923

Purpose

SOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

SOX10 promoter targeting gRNA

Use

Lentiviral

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Px458-Cas9n-Trp73-Exon4-3sgRNA

Plasmid

#88850

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
Px458-Cas9n-Trp73-Exon4-4sgRNA

Plasmid

#88851

Purpose

CRISPR KO of Trp73

Depositor

Joan Massague

Article

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp73 (Trp73 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only
XE125 XDsh delta COOH-GFP-CS2+

Plasmid

#16784

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-COOH (dvl2.L Frog)

Use

Xenopus expression

Tags

GFP

Mutation

contains Dsh amino acids 1-588 (deleting 599-736)

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
Flag-NHR2+R pcDNA3.1

Plasmid

#17317

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
NHR1+2-Flag pcDNA3.1

Plasmid

#17316

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pGS1212

Plasmid

#63785

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

eGFP-Strep-tag II followed by URA3 selection marker

Use

E. coli cloning vector

Tags

eGFP-Strep-tag II

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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PB-CAG-HRKI(Haunt P-Inr2-cDNA KI)

Plasmid

#92163

Purpose

For lncRNA CAG promoter homologue recombination mediated knockin

Depositor

Xiaohua Shen

Article

Yin et al Cell Stem Cell. 2015 May 7;16(5):504-16.

Insert

Haunt CAG KI homology arms

Use

Other

Available Since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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