We narrowed to 20,263 results for: Sele
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Plasmid#214674PurposeMAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exonsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAPT (MAPT Human)
TagsFirefly Luciferase, Renilla Luciferase, and V5ExpressionMammalianMutationContains Exon 9 and Exon 11, with a BamHI EcoRI c…PromoterCMVAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-888 USP7
Plasmid#131248PurposeMammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLINC-AKAR1(TA)-CAAX
Plasmid#87706PurposeNegative-control mutant of FLINC-AKAR1-CAAX.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLINC-AKAR1(TA)-CAAX
Tags6xHis, C-terminal targeting sequence from Kras, T…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-LacI
Plasmid#103814PurposeBLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL2 (NT931)
Plasmid#49063PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #2 and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL2 inserted at aa398 in hNKCC1 …PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE
Plasmid#196414PurposeAstrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smFLAG
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianPromoterCAG and GfaABC1DAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-Centrobin
Plasmid#136829PurposeMammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSNAP-Centrobin (CNTROB Synthetic, Human)
TagsFLAG-tag / His-tagExpressionMammalianPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
ORAI1-YFP L273D-L276D
Plasmid#114182PurposeORAI1 channel with C-terminal YFP, carrying the double mutation L273D-L276D in the STIM1 binding domain, leading to a channel gating deficiency.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertORAI1-YFP L273D-L276D (ORAI1 Human)
TagsYFPExpressionMammalianMutationL273D/L276DAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLXEBR-pTF-Ngn3
Plasmid#114307PurposeTET-inducible expression of the Neugenin 3 transcription factorDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA5
Plasmid#64985PurposeExpresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, D160N, D165A, K19…Promotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHSP70B-CP6-TK
Plasmid#165881PurposeBasic ganciclovir or acyclovir sensitvity cassette in Alpha2, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSP70B-CP6-FCU1
Plasmid#165882PurposeBasic 5-fluorocytosine sensitivity cassette in Alpha2, lacks physical marker or attB siteDepositorInsertBasic Selection Vector
UseSynthetic BiologyAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGyA
Plasmid#160292PurposeDrives expression of Cas9 and a TdTomato fluorescent markerDepositorInsertsVas promoter
hSpCas9
tdTomato
CTCF insulator
Gypsy insulator
Left homology arm Y chromosome
Right homology arm Y chromosome
TagsT2A GFPExpressionInsectAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PDE4D2cat-ICUE4
Plasmid#181847PurposeICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagsECFP, PDE4D2(86-418), and cpVenus(L194)ExpressionMammalianMutationGlutamine 122 in Epac1 domain mutated to glutamat…PromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1
Plasmid#184398PurposeDonor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla luciferase beta-globin-PTC39
UseCRISPR; Donor plasmid for hdrTags3X FLAGExpressionMammalianMutationPTC in beta-globin at amino acid position 39PromoterTet-On 3GAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
R620-M14-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.351
Plasmid#170198PurposeMammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
TagsHis6ExpressionMammalianMutationL18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…PromoterCMV51Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 HA-ECL4 (NT935)
Plasmid#49065PurposeExpresses human NKCC1 mutant with HA tag inserted into extracellular loop #4 and N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutation2xHA epitope in ECL4 inserted at aa574 in hNKCC1 …PromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-MCS-LexA-VP16-MiniWhite
Plasmid#165898PurposePuromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3(C504A)
Plasmid#39485DepositorPublicationInsertARAP3(C504A) (ARAP3 Human)
TagsEGFPExpressionMammalianMutationC504A = Arf GAP domain mutationAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFudio-Cpg15-IRES2-TdTomatoW
Plasmid#134309PurposeExpresses cpg15 and TdTomato upon coexpression with Cre recombinaseDepositorInsertsUseCre/Lox and LentiviralExpressionMammalianPromoterUbCAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only