165,688 results

1. pMXS-IRES-BLAST flag-CAD

   Plasmid

   #169818

   Purpose

   Expresses C-terminal flag-tagged CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   TCCC -> AGTC silent mutations at nt527-530 eli…

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHTN_HaloTag_KIF5B

   Plasmid

   #213404

   Purpose

   Full-length human kinesin-1 with HaloTag at the N-terminus

   Depositor

   Jonas Ries

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   HaloTag-KIF5B (KIF5B Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-mNRDc-V5

   Plasmid

   #51297

   Purpose

   expression of mouse NRDc in mammalian cells

   Depositor

   Eiichiro Nishi

   Article

   Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

   Insert

   NRDc (Nrd1 Mouse)
   

   Tags

   HIS and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. qTAG-C-mScarlet-EFS-Puro

   Plasmid

   #227259

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAF020 p3E-2A-mCerulean

   Plasmid

   #195974

   Purpose

   Gateway p3E 2A fluorophore

   Depositor

   Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   2A-mCerulean
   

   Use

   Other

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Flag-hCRM1

   Plasmid

   #17647

   Depositor

   Xin Wang

   Article

   Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

   Insert

   CRM1 (XPO1 Human)
   

   Tags

   3x Flag

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3-PAX3-FOXO1/FKHR

   Plasmid

   #115526

   Purpose

   Mammalian expression construct

   Depositor

   Martine Roussel

   Article

   Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

   Insert

   Pax3-FOXO1/FKHR (FOXO1, Pax3 Human, Mouse)
   

   Expression

   Mammalian

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1_ 2xFLAG-2xSTREP_KEAP1

   Plasmid

   #159128

   Purpose

   expresses KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   KEAP1 (KEAP1 Human)
   

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1/ZMYM2-FGFR1

   Plasmid

   #167486

   Purpose

   Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

   Depositor

   Nicholas Cross

   Article

   Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

   Insert

   ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
   

   Expression

   Mammalian

   Available Since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENN496_miniCRISPRcharm_dSaCas9

    Plasmid

    #220837

    Purpose

    mini CRISPRcharm expression in mammalian cells

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    mini CRISPRcharm
    

    Use

    CRISPR

    Tags

    P2A-TagBFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HER2 CA (V659E)

    Plasmid

    #16259

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Expression

    Mammalian

    Mutation

    Constitutively active: V659E

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHCMV_FLUAV_HA

    Plasmid

    #207273

    Purpose

    Mammalian expression of Influenza A virus HA protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUAV_HA (HA Influenza A virus)
    

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-PGK-EGFP-ALDH1A2-Neo

    Plasmid

    #189743

    Purpose

    The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A2 (ALDH1A2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 460 p3E-2A-mCherrypA

    Plasmid

    #195970

    Purpose

    Gateway p3E 2A fluorophore

    Depositor

    Christian Mosimann, Kristen Kwan

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    2A-mCherry
    

    Use

    Other

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. KG#908

    Plasmid

    #110914

    Purpose

    Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans

    Depositor

    Kenneth Miller

    Inserts

    rab-3 promoter
    TIR1 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD103

    Plasmid

    #119887

    Purpose

    Constitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

    Insert

    pConstitutive_mCherry
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRetroX-PTuner DD-linker-M.EcoGII

    Plasmid

    #122082

    Purpose

    Express M.EcoGII with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of M.EcoGII

    Expression

    Bacterial and Mammalian

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMOS014: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163055

    Purpose

    Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge