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ERrefGFP1_pCDNA3

Plasmid

#48635

Purpose

Mam vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with mut Cys147 to Ser, del insertion147b and mut Cys204 to Glut.Referred to as ref GFP

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERrefGFP1

Use

Tags

FLAG

Expression

Mammalian

Mutation

C147S,C204Q

Promoter

Available since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-aDTX-lumitoxin

Plasmid

#51686

Purpose

Expression vector for aDTX-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

aDTX-lumitoxin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-DTXK-lumitoxin

Plasmid

#51691

Purpose

Expression vector for DTXK-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

DTXK-lumitoxin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CONK1-lumitoxin

Plasmid

#51689

Purpose

Expression vector for CONK1-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

CONK1-lumitoxin

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRPV1exCellHalo

Plasmid

#220307

Purpose

Mammalian expression vector for a subunit of rat TRPV1 with circularly permutated HaloTag inserted between the S5 and S6 transmembrane helices

Depositor

Eric Senning

Article

Mott et al J Gen Physiol. 2024 Oct 7;156(10):e20231

Insert

rTRPV1exCellHalo (Trpv1 Rat)

Use

Tags

circularly permutated Halotag (Deo et al., Nat Ch…

Expression

Mammalian

Mutation

linker included to insert cpHaloTag (

Promoter

CMV promoter

Available since

July 30, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 NL

Plasmid

#113442

Purpose

NanoLuc control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

NanoLuc

Use

Luciferase

Tags

cmyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mAMCase_CatD_6xHis_pcDNA3.1

Plasmid

#200229

Purpose

Expresses mouse Acidic Mammalian Chitinase catalytic domain with C-terminal 6xHis tag in pcDNA 3.1 vector

Depositor

James S Fraser

Article

Diaz et al Elife. 2024 Jun 17;12:RP89918. doi: 10.7

Insert

Acidic Mammalian Chitinase (Chia1 Mouse)

Use

Tags

6xHis

Expression

Mammalian

Mutation

S354F

Promoter

CMV

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRXRα

Plasmid

#135910

Purpose

Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

Human retinoid X receptor- alpha (RXRA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARα.hRXRα

Plasmid

#135411

Purpose

Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-alpha
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-NORAD

Plasmid

#120383

Purpose

Expression vector for lncRNA NORAD

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

NORAD (NORAD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARγ.hRXRα

Plasmid

#135416

Purpose

Human Retinoic Acid Receptor-gamma and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-gamma
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hRARβ.hRXRα

Plasmid

#135415

Purpose

Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Inserts

Human retinoic acid receptor-beta
Human retinoid X receptor-alpha

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/EV

Plasmid

#120833

Purpose

Control vector, 20nt random sequence between gateway recombination sites

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-NL

Plasmid

#113445

Purpose

ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hHNF4alpha

Plasmid

#206878

Purpose

The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.

Depositor

Catharine Ross, Reza Zolfaghari

Article

Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

Insert

Human hepatocyte nuclear factor 4 alpha (HNF4A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 WT

Plasmid

#222653

Purpose

Mammalian expression vector to express mouse Regnase-1 WT

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-3 WT

Plasmid

#222655

Purpose

Mammalian expression vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-Flag

Plasmid

#210342

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

Flag tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PcDNA3.1-2Strep

Plasmid

#210343

Purpose

Empty Vector for molecular cloning

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

2Strep tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Gβγ-BERKY3

Plasmid

#158219

Purpose

BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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