12,483 results

1. pCAG-Kir2.1-T2A-tdTomato

   Plasmid

   #60598

   Purpose

   Expresses Kir2.1-T2A-tdTomato

   Depositor

   Massimo Scanziani

   Article

   Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

   Insert

   Kir2.1-T2A-tdTomato (Kcnj2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBITE-CD19-CD3

   Plasmid

   #190677

   Purpose

   Encodes a modularized form of HD37-OKT3 bispecific antibody

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Modularized-HD37-modularized-OKT3-P2A-NeonGreen
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-flex-Unc5cHA-WPRE-SV40pA

   Plasmid

   #205442

   Purpose

   production of AAV for cre-dependent expression of Unc5C, tagged with 3xHA at C-terminus

   Depositor

   Jeremy Kay

   Article

   Prigge et al Dev Cell. 2023 Oct 23;58(20):2080-2096.e

   Insert

   Unc5c (Unc5c Mouse)
   

   Use

   AAV

   Tags

   3xHA

   Expression

   Mutation

   Promoter

   mini-CMV (with MVM small intron)

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRX-mSTING-EGFP

   Plasmid

   #214148

   Purpose

   Retroviral transduction of EGFP-tagged STING

   Depositor

   Nan Yan

   Article

   Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

   Insert

   STING (Sting1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GAD67 scFv [L127/8]

   Plasmid

   #190509

   Purpose

   Mammalian Expression of GAD67 scFV. Derived from hybridoma L127/8.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   GAD67 (Homo sapiens) recombinant scFV (GAD1 Mouse)
   

   Use

   Tags

   Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-sgR26

   Plasmid

   #127376

   Purpose

   Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   ROSA26 (Gt(ROSA)26Sor Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-EF1-mVenus-p27K−

   Plasmid

   #176651

   Purpose

   It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

   Depositor

   Mohamed Bentires-Alj

   Article

   Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

   Insert

   mVenus-p27K- (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mutation

   Mutant K- (lacks affinity to Cdk)

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

   Plasmid

   #175549

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   WAPL (Wapl Mouse)
   

   Use

   Mouse Targeting

   Tags

   AID[71-114]-eGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Xist-PA

   Plasmid

   #26760

   Depositor

   Rudolf Jaenisch

   Article

   Wutz et al Mol Cell. 2000 Apr . 5(4):695-705.

   Insert

   Xist (Xist Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. anti-FLAG M2 light chain

    Plasmid

    #175358

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 light chain

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 light chain
    

    Use

    Tags

    N-terminal secretion signal peptide (MEAPAQLLFLLL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. anti-FLAG M2 heavy chain

    Plasmid

    #175359

    Purpose

    Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

    Depositor

    Joost Snijder

    Article

    Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

    Insert

    anti-FLAG M2 heavy chain
    

    Use

    Tags

    His8 tag and N-terminal secretion signal peptide …

    Expression

    Mammalian

    Mutation

    L51I (see paper)

    Promoter

    CMV

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. gag-COM-pol

    Plasmid

    #211373

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MMLVgag-COM-pol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP Sec22b

    Plasmid

    #101918

    Purpose

    Expresses Sec22b in mammalian cells

    Depositor

    Thierry Galli

    Article

    Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. do

    Insert

    Sec22b (Sec22b Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pR26 CAG AsiSI/MluI

    Plasmid

    #74286

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPCX-VEcadTS

    Plasmid

    #45848

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin Tension Sensor (Cdh5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. DV-GFP-Rab38-WT-pDEST53

    Plasmid

    #15669

    Depositor

    William Pavan

    Article

    Loftus et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99

    Insert

    Rab38 (Rab38 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    wildtype Rab38 sequence

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-Pom121 GFP

    Plasmid

    #187680

    Purpose

    In vivo visualization of NPC protein Pom121

    Depositor

    Mary Beckerle

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. qi5

    Plasmid

    #24501

    Depositor

    Bruce Conklin

    Article

    Conklin et al Nature. 1993 May 20. 363(6426):274-6.

    Insert

    Gq alpha (Gnaq Mouse)
    

    Use

    Tags

    HA (see comments)

    Expression

    Mammalian

    Mutation

    Gq alpha with the C-terminal amino acids changed …

    Promoter

    Available since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-N1-mSNX27

    Plasmid

    #163617

    Purpose

    mouse SNX27 cDNA N-terminally tagged with GFP

    Depositor

    Josef Kittler

    Article

    Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

    Insert

    mouse SNX27 (Snx27 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. P4-PE

    Plasmid

    #211375

    Purpose

    Packaging plasmid for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    P4-PE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge