Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 536 results for: M10

Showing: 161 - 200 of 536 results

  1. pSCM167-Venus-VF-hADAM10(aa455-654)

    Plasmid
    #189561
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only disintegrin and cysteine rich domains--amino acids 455-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa 455-654
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    189561-plasmid-map-sequence-id-374541

    Enlarge

  7. pSCM167.2-Venus-VF-hADAM10(aa1-654)

    Plasmid
    #189558
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain through cysteine rich domain--amino acids 1-654
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    VF
    Expression
    Mammalian
    Mutation
    contains aa1-654
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189558-plasmid-map-sequence-id-374390

    Enlarge

  8. pJM103: 150bp Hps2 RFP on pCM66T

    Plasmid
    #74744
    Purpose
    pCM66T with shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pHps2, trimmed (extracted from Methylovorus gluco…
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74744-plasmid-map-sequence-id-141459

    Enlarge

  10. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human

    Plasmid
    #238130
    Purpose
    This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238130-plasmid-map-sequence-id-476571

    Enlarge

  11. pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

    Plasmid
    #189562
    Purpose
    Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213
    Depositor
    Haian Fu
    Insert
    truncated ADAM metallopeptidase domain 10 (ADAM10 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa 1-213
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    189562-plasmid-map-sequence-id-374127

    Enlarge

  13. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Monkey

    Plasmid
    #238132
    Purpose
    This plasmid encodes the full-length African Green Monkey (Chlorocebus sabaeus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238132-plasmid-map-sequence-id-476568

    Enlarge

  14. pLCKO-CMV-TMEM106B-M210A_F213A-P2A-Blasticidin

    Plasmid
    #237479
    Purpose
    Encodes full-length human TMEM106B. M210A/F213A mutations block S1 binding and SARS-CoV-2 Belgium/GHB-03021/2020 TMEM106B mediated infection, without affecting TMEM106B expression or localization.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (TMEM106B Human)
    Use
    Lentiviral
    Tags
    P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    M210A + F213A
    Promoter
    CMV
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    237479-plasmid-map-sequence-id-476565

    Enlarge

  15. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

    Plasmid
    #238131
    Purpose
    This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238131-plasmid-map-sequence-id-476564

    Enlarge

  16. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Mouse

    Plasmid
    #238133
    Purpose
    This plasmid encodes the full-length Mouse (Mus musculus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B (Tmem106b Mouse)
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238133-plasmid-map-sequence-id-476563

    Enlarge

  19. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid
    #118259
    Purpose
    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118259-plasmid-map-sequence-id-228500

    Enlarge

  27. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

    Enlarge

  28. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid
    #228939
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228939-plasmid-map-sequence-id-469416

    Enlarge

  29. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid
    #233345
    Purpose
    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    BAX(3-171), N-terminus and C-terminus are deleted…
    Promoter
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233345-plasmid-map-sequence-id-469414

    Enlarge

  31. pLCKO-CMV-SARS-CoV-2-S-Bel-P5_5-7-IRES-mNeonGreen-NES/PKI-P2A-Blasticidin

    Plasmid
    #237480
    Purpose
    Encodes full-length SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate), featuring the E484D substitution, which likely emerged during passaging and enhances TMEM106B binding.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    SARS-CoV-2 Spike protein (Belgium/GHB-03021 isolate)
    Use
    Lentiviral
    Tags
    IRES-mNeonGreen-NES/PKI and P2A-Blasticidin
    Expression
    Mammalian
    Mutation
    S484D + S813I + deleted amino acids 68-76 + 676-6…
    Promoter
    CMV
    Available since
    July 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    237480-plasmid-map-sequence-id-477747

    Enlarge

  32. T4 Lysozyme Core 10

    Plasmid
    #18424
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V87I I100V M102L V103I M106I V111A M120Y L133F V1…
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only

  33. T4 Lysozyme Core 10 A111V

    Plasmid
    #18425
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V87I I100V M102L V103I M106I M120Y L133F V149I T1…
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only

  34. T4 Lysozyme Core 10 I103V

    Plasmid
    #18426
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    V87I I100V M102L M106I V111A M120Y L133F V149I T1…
    Promoter
    Available since
    Aug. 28, 2008
    Availability
    Academic Institutions and Nonprofits only

  35. pHL1948

    Plasmid
    #53046
    Purpose
    PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHindM10:RBS(st3):csrA
    Depositor
    Han Lim
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pConNoHind, pConNoHindM10, and pConNoHindM12
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 161 - 200 of 536 results