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Showing: 161 - 200 of 548 results

  5. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Use
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

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  9. Opto-beta2AR

    Plasmid
    #106063
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-beta2AR
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106063-plasmid-map-sequence-id-209702

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  10. Opto-alpha1AR

    Plasmid
    #106064
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-alpha1AR
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106064-plasmid-map-sequence-id-209703

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  12. Opto-D2R

    Plasmid
    #106068
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D2R; Opto-D2R; F10; 721)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-D2R
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106068-plasmid-map-sequence-id-232402

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  13. Opto-M2R

    Plasmid
    #106070
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M2R; Opto-M2R; F12; 723)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-M2R
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106070-plasmid-map-sequence-id-209712

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  15. Opto-M3R

    Plasmid
    #106071
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-M3R; Opto-M3R; H1; 724)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-M3R
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106071-plasmid-map-sequence-id-209714

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  19. Opto-D1R

    Plasmid
    #106067
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-D1R; Opto-D1R; F9; 720)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-D1R
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106067-plasmid-map-sequence-id-209709

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  20. pLenti-gRNA-puro

    Plasmid
    #180426
    Purpose
    Expresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.
    Depositor
    Moshe Szyf
    Article
    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:
    Insert
    gRNAscr
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    180426-plasmid-map-sequence-id-354805

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  21. pHCas9-Nours

    Plasmid
    #107733
    Purpose
    Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    Cas9
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Human optimized
    Promoter
    TEF1
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107733-plasmid-map-sequence-id-208542

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  23. Opto-FFR3

    Plasmid
    #106065
    Purpose
    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-FFR3; Opto-FFR3; F7; 718)
    Depositor
    Harald Janovjak
    Article
    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:
    Insert
    Opto-FFR3
    Use
    Tags
    Rhodopsin-1D4 and VSV-G
    Expression
    Mammalian
    Mutation
    Chimeric receptor protein
    Promoter
    CMV
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    106065-plasmid-map-sequence-id-209706

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  25. pV5endoNA2

    Plasmid
    #174729
    Purpose
    Expresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    V5 epitope tag
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174729-plasmid-map-sequence-id-343520

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  30. pDT386

    Plasmid
    #174733
    Purpose
    Expresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    DT386
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Deleted amino acids 412-560 (the receptor binding…
    Promoter
    T5
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174733-plasmid-map-sequence-id-343525

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  37. pYES2-gRNA-hyg-MCS

    Plasmid
    #107734
    Purpose
    E. coli-S. cerevisiae shuttle plasmid harbors gRNA expression cassettes targeting S. cerevisiae chromosomal X-3 site (HXK1 gene). Can function as a gRNA expression empty backbone plasmid.
    Depositor
    Sheng Yang
    Article
    Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.
    Insert
    X-3 site gRNA expression cassettes
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    SNR52p
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    107734-plasmid-map-sequence-id-208539

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  40. pFendoNA2S

    Plasmid
    #174730
    Purpose
    Expresses GFP-endoNA2-S706A fusion protein for detection of polysialic acid. The endoNA2-S706A is a noncatalytic endosialidase that lacks the ability for releasing its C-terminal chaperone domain.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    endoNA2-S706A
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed Serine 706 to Alanine
    Promoter
    Available since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174730-plasmid-map-sequence-id-343521

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Showing: 161 - 200 of 548 results