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We narrowed to 10,794 results for: T7

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Showing: 161 - 200 of 10794 results

  7. T7 promoter + terminators reporter plasmid

    Plasmid
    #156456
    Purpose
    A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter and a terminator array.
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560
    Inserts
    NeoR/KanR
    TcR
    Expression
    Bacterial
    Mutation
    ATG start codon mutated to ACG
    Promoter
    Em7 and tet
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156456-plasmid-map-sequence-id-311167

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  9. pUC57kan-T7-gRNA-U6 V2

    Plasmid
    #115520
    Purpose
    To construct multiple sgRNA expression vector for CRISPR
    Depositor
    Xingxu Huang
    Insert
    sgRNA scaffolf-U6 promoter
    Use
    CRISPR
    Available Since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    115520-plasmid-map-sequence-id-224556

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  13. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  14. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  18. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid
    #171660
    Purpose
    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171660-plasmid-map-sequence-id-339032

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  19. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

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  22. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  23. pBJG1 T7-8xHis-OGT 5N5A

    Plasmid
    #154280
    Purpose
    Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; bact…
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154280-plasmid-map-sequence-id-304840

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  29. pCDNA3 T7 Jip4 (1-272)

    Plasmid
    #58928
    Purpose
    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
    Depositor
    Roger Davis
    Article
    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.
    Insert
    Mapk8ip4 (Spag9 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Jip4 (1-272aa)
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. pCDNA3 T7 Jip4 (1-151)

    Plasmid
    #58926
    Purpose
    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
    Depositor
    Roger Davis
    Article
    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.
    Insert
    Mapk8ip4 (Spag9 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Jip4 (1-151aa)
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  31. pCDNA3 T7 Jip4 (1-212)

    Plasmid
    #58927
    Purpose
    JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6
    Depositor
    Roger Davis
    Article
    Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.
    Insert
    Mapk8ip4 (Spag9 Mouse)
    Tags
    T7
    Expression
    Mammalian
    Mutation
    Jip4 (1-212aa)
    Available Since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. pGGSG-Bromo-T7-P2a-Neo

    Plasmid
    #235516
    Purpose
    Cloning vector for GGSG-BromoTag-T7-P2A-Neo. Clone into ZraI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, neo
    Mutation
    None
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235516-plasmid-map-sequence-id-474044

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  35. pGGSG-Bromo-T7-T2a-Pac

    Plasmid
    #235518
    Purpose
    Cloning vector for GGSG-BromoTag-T7-T2A-Pac. Clone into PmlI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Puro
    Mutation
    None
    Available Since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    235518-plasmid-map-sequence-id-474042

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  36. pGGSG-Bromo-T7-T2a-Bsd

    Plasmid
    #235519
    Purpose
    Cloning vector for GGSG-BromoTag-T7-T2A-Bsd. Clone into ZraI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Bsd
    Mutation
    None
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235519-plasmid-map-sequence-id-474008

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  37. pGGSG-Bromo-T7-P2a-Pac

    Plasmid
    #235517
    Purpose
    Cloning vector for GGSG-BromoTag-T7-P2A-Pac. Clone into PmlI and AfeI
    Depositor
    Brian Hendrich
    Insert
    BromoTag
    Use
    Mouse Targeting
    Tags
    BromoTag, T7, Puro
    Mutation
    None
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235517-plasmid-map-sequence-id-473996

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Showing: 161 - 200 of 10794 results