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We narrowed to 220 results for: sleeping beauty

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Showing: 161 - 200 of 220 results

  2. pAF197

    Plasmid
    #135930
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    CTLA4 fused to IgG Fc and to mCherry
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135930-plasmid-map-sequence-id-270077

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  3. pAF139

    Plasmid
    #135924
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mTagBFP
    Use
    Transposon
    Tags
    mTagBFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135924-plasmid-map-sequence-id-270080

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  5. pT/CAGGS-NRASG12V/D38A-IRES-mVenus

    Plasmid
    #236077
    Purpose
    Sleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and a double mutant NRAS (NRASG12VD38A).
    Depositor
    Masashi Narita
    Article
    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:
    Inserts
    mVenus
    NRAS G12V D38A (NRAS Human)
    Use
    Sleeping beauty transposon plasmid
    Mutation
    G12V D38A
    Promoter
    CAGGS
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236077-plasmid-map-sequence-id-472893

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  7. pAF138

    Plasmid
    #135923
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCitrine
    Use
    Transposon
    Tags
    mCitrine
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135923-plasmid-map-sequence-id-270125

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  8. pAF164

    Plasmid
    #135928
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mOrange
    Use
    Transposon
    Tags
    mOrange
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135928-plasmid-map-sequence-id-270165

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  9. pT3EF1aH-myr-Akt

    Plasmid
    #179909
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.
    Depositor
    Xin Chen
    Article
    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:
    Insert
    Akt1 (Akt1 Mouse)
    Tags
    HA and Myr
    Expression
    Mammalian
    Available Since
    April 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    179909-plasmid-map-sequence-id-357764

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  10. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  11. pSBtet-2xMARS-nGFP-puro

    Plasmid
    #205239
    Purpose
    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Transposon
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFPā€¦
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank referenceā€¦
    Promoter
    tight TRE promoter
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205239-plasmid-map-sequence-id-404740

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  13. pAF160

    Plasmid
    #135925
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mAzurite
    Use
    Transposon
    Tags
    mAzurite
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135925-plasmid-map-sequence-id-270082

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  14. pAF161

    Plasmid
    #135926
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mCerulean3
    Use
    Transposon
    Tags
    mCerulean3
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135926-plasmid-map-sequence-id-270079

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  15. pAF163

    Plasmid
    #135927
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    TNFRSF9 fused to mNeptune2
    Use
    Transposon
    Tags
    mNeptune2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135927-plasmid-map-sequence-id-270084

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  20. pAF92

    Plasmid
    #135929
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Insert
    mCherry fused to TNFSF9
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135929-plasmid-map-sequence-id-270167

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  23. pLP-PIGA3 (pLP305)

    Plasmid
    #196992
    Purpose
    Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.
    Depositor
    Matthew Maurano
    Article
    Brosh et al Mol Cell. 2023 Apr 6;83(7):1140-1152.e7.
    Type
    Empty backbone
    Use
    Cre/Lox, Mouse Targeting, and Synthetic Biology ;ā€¦
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    196992-plasmid-map-sequence-id-389805

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  27. pT3-EF1aH-PIK3CA H1047R

    Plasmid
    #180031
    Purpose
    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.
    Depositor
    Xin Chen
    Article
    Wang et al Liver Int. 2015 Dec 30. doi: 10.1111/liv
    Insert
    PIK3CA H1047R (PIK3CA Human)
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    H1047R
    Promoter
    EF1a
    Available Since
    March 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180031-plasmid-map-sequence-id-357864

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  38. pKT-IDH1(R132H)-IRES-Katushka

    Plasmid
    #124257
    Purpose
    Expresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii
    Insert
    Isocitrate dehydrogenase 1 (R132H) (IDH1 Human)
    Expression
    Mammalian
    Mutation
    Arginine 132 is mutated to Histidine. This mutatiā€¦
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124257-plasmid-map-sequence-id-252091

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  40. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Depositor
    Donna Romero, Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Promoter
    pTRE bidirectional tet-on
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213031-plasmid-map-sequence-id-422025

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Showing: 161 - 200 of 220 results