We narrowed to 296 results for: actb

1. pBS-ACTB-C200-GFPfr2

   Plasmid

   #170726

   Purpose

   HR donor vector for ACTB gene with the revised marker sequence for ASHRA.

   Depositor

   Natsuko Chiba

   Insert

   Donor sequence to ACTB gene for HR with the revised marker sequence
   

   Use

   Hr donor vector for human actb gene.

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Puro-mScarlet-ACTB

   Plasmid

   #207754

   Purpose

   Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pX459-HypaCas9-mR2-ACTB_sgRNA

   Plasmid

   #183884

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB sgRNA spacer (ACTB Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZc-Ub-R-HsACTB*-thymosinB-8His

   Plasmid

   #111149

   Purpose

   Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

   Depositor

   Mohan Balasubramanian

   Article

   Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

   Insert

   ACTB (ACTB Human)
   

   Use

   Pichia pastoris integration plasmid

   Tags

   Thymosin beta and a His-tag

   Expression

   Yeast

   Mutation

   N-terminal arginylation

   Promoter

   AOX1

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS23.U6<ActB.mus_C-term-KI_gRNA

   Plasmid

   #207408

   Purpose

   gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   ActB
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNanog-Halo-Ty2-BactBsd

   Plasmid

   #235511

   Purpose

   C-term HALO targeting construct

   Depositor

   Brian Hendrich

   Insert

   Nanog (Nanog Mouse)
   

   Use

   Mouse Targeting

   Tags

   HALO, 2xTy

   Mutation

   None

   Promoter

   beta actin

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pS12<ActB.mus_2A-mCh_KI-HMEJ

   Plasmid

   #207407

   Purpose

   Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   Actb (Actb Mouse)
   

   Use

   CRISPR and Mouse Targeting; Hmej knockin donor

   Tags

   mCherry

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Solo-mScarlet-ACTB

   Plasmid

   #207750

   Purpose

   Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. qTAG-N-Solo-mNeon-ACTB

   Plasmid

   #207749

   Purpose

   Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMMK ENTR 1 HITI-2c ACTB

    Plasmid

    #206269

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HITI-2c donor
    

    Use

    CRISPR; Multimate/gateway entr 1

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKI-cjACTB-EGFP-3'short

    Plasmid

    #129749

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKI-cjACTB-EGFP-5'short

    Plasmid

    #129748

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. His-FLAG-Tev_ACTB (beta-actin)

    Plasmid

    #188453

    Purpose

    Express in mammalian cells human beta-actin (ACTB) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTB (ACTB Human)
    

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

    Plasmid

    #227775

    Purpose

    Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    BFP-MCS
    mCherry-t2A-CreERT2
    

    Use

    Zebrafish expression

    Promoter

    Olactb and hsp70l

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ6332 - ACTB CRISPRa CLIP Donor

    Plasmid

    #210015

    Purpose

    CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    dLbCpf1-miniVPR-T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. actb2-SecAnxaV-mKate2-acry-EGFP

    Plasmid

    #123628

    Purpose

    Ubiquitous zebrafish expression vector containing secreted human AnnexinV tagged to mKate2. Parton lab clone BDN.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    AnnexinA5
    

    Tags

    mKate2

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPC-mCh-ACTB-P2A-EGFP

    Plasmid

    #165158

    Purpose

    Expresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cells

    Depositor

    Taekjip Ha

    Article

    Zou et al Mol Cell. 2021 Apr 1;81(7):1553-1565.e8.

    Insert

    mCherry-ACTB-P2A-EGFP (ACTB Human)
    

    Use

    Retroviral

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKI-cjACTB-EGFP-5'3'short

    Plasmid

    #129750

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    ACTB (ACTB C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    EGFP

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHnS KI;Actb Donor;3xV5 KO;Template

    Plasmid

    #240291

    Purpose

    KI:Actb Donor:3xV5 KO:Template

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Actb
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHnS KI;Actb Donor;3xV5 KO;Dlg4

    Plasmid

    #240292

    Purpose

    KI:Actb Donor:3xV5 KO:Dlg4

    Depositor

    Yuki Ogawa

    Insert

    KI gRNA for Actb
    

    Use

    AAV

    Mutation

    NA

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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