We narrowed to 2,056 results for: ENDOG

1. pX330-RAB7A

   Plasmid

   #227297

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of RAB7A (RAB7A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCMV-AID-PcrA-UGI-T2A-mTagBFP2

   Plasmid

   #221224

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase with mTagBFP2 reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI-T2A-mTagBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Circular 200,100 with interspersed loops (FANCC)

   Plasmid

   #180192

   Purpose

   AAV vector carrying a guide RNA targeting the human FANCC mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Circular 200,100 with interspersed loops (CTNNB1)

   Plasmid

   #180193

   Purpose

   AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA with interspersed loops
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. (364) SB KRAB-SadCas9

   Plasmid

   #163022

   Purpose

   Sleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   Transposon

   Tags

   KRAB and myc NLS

   Expression

   Mammalian

   Promoter

   TET-On

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pODN-AAVS1-mNG-CAAX

   Plasmid

   #183870

   Purpose

   Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mNeonGreen-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. qTAG-N-Solo-mStayGold-MYO1C

   Plasmid

   #227306

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAC149-pCR8-dCas9VP160

   Plasmid

   #48221

   Purpose

   dCas9VP160 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP160

   Mutation

   D10A;H840A nuclease-deficient

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LADL Anchor

    Plasmid

    #127664

    Purpose

    Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    dCas9-CIBN-2A-Puro
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 (D10A,H840A) mutant

    Promoter

    EF1a

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459-HypaCas9-ANLN_sgRNA

    Plasmid

    #183874

    Purpose

    pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

    Plasmid

    #192160

    Purpose

    Dual AAV vector AAV-dCas9

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Insert

    Dead Cas9
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47455

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Use

    AAV

    Tags

    2A_GFP

    Expression

    Mammalian

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX459-HypaCas9-ECT2_sgRNA

    Plasmid

    #183873

    Purpose

    pX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ECT2 sgRNA spacer (ECT2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAC147-pCR8-dCas9VP64

    Plasmid

    #48219

    Purpose

    dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR; Gateway donor

    Tags

    HA Tag and VP64

    Mutation

    D10A;H840A

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSNR52-sgTET

    Plasmid

    #46923

    Purpose

    Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    sgRNA targeting endogenous TRE elements of pTET07 promoter
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pODN-AAVS1-mR2-CAAX

    Plasmid

    #183871

    Purpose

    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-CAAX

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Promoter

    CMV

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX458-GFP-TRIM28

    Plasmid

    #185010

    Purpose

    Encodes gRNA for mouse TRIM28 along with Cas9 with 2A-EGFP

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    TRIM28 sgRNA (Trim28 Mouse)
    

    Use

    CRISPR

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-gfap-sgRNA

    Plasmid

    #65566

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish gfap locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    gfap sgRNA (gfap Zebrafish)
    

    Use

    In vitro rna transcription

    Promoter

    T7

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge