164,577 results

1. pcDNA3.1(+) K-Ras(G12C)

   Plasmid

   #224284

   Purpose

   Cell transfection and expression of K-Ras (G12C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   K-Ras (KRAS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12C

   Promoter

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-zTRAP

   Plasmid

   #42236

   Depositor

   Stephen L Johnson

   Article

   Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

   Insert

   EGFP-Rpl10a (rpl10a Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-mStayGold-EFS-Zeo

   Plasmid

   #227258

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK7-HA-S6K1-E389-deltaCT

   Plasmid

   #8993

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T389E, stop codon at amino acid 402

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1-SA-2A-NEO-CAG-RTTA3

   Plasmid

   #60431

   Purpose

   neomycin resistance; constitutive promoter driving reverse tet activator

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   rtTA3
   

   Use

   Zfn donor

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-hsRPA14 (MSW#503)

   Plasmid

   #208069

   Purpose

   Expression of GFP-tagged hsRPA3 in human cells

   Depositor

   Marc Wold

   Article

   Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

   Insert

   RPA3 (RPA3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBC-MT2T3

   Plasmid

   #50594

   Purpose

   CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

   Depositor

   Qi-Jun Chen

   Article

   Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

   Insert

   T2, gRNA scaffold, TaU3t plus, U6-26p, T3
   

   Use

   CRISPR; Pcr template

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-Talin

   Plasmid

   #80026

   Purpose

   Mammalian expression of mCherry-Talin

   Depositor

   Chinten James Lim

   Article

   Lee et al Mol Biol Cell. 2013 May;24(9):1354-62. d

   Insert

   Talin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-NES-ZapCV2-Zinc dead

    Plasmid

    #203850

    Purpose

    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWS175

    Plasmid

    #90962

    Purpose

    Cas9 gap repair vector - HygR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBJ2

    Plasmid

    #167131

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Kanamycin promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid

    #227327

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-hsRPA32 (MSW#497)

    Plasmid

    #208068

    Purpose

    Expression of GFP-tagged hsRPA2 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

    Insert

    RPA2 (RPA2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T88I- please see depositor comments

    Promoter

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTagBFP-C1-FKBP-PIP5K1C-HD-EE

    Plasmid

    #220090

    Purpose

    Recruitable PIP5K to make PIP2

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PIP5K1C (PIP5K1C Human)
    

    Use

    Tags

    TagBFP-FKBP1A(3-108)

    Expression

    Mammalian

    Mutation

    amino acids 1-635; D101R, R304D, R445E, K446E

    Promoter

    CMV

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-NLS-ddMisCas13b-SNAPf-NLS-3xFlag

    Plasmid

    #191537

    Purpose

    Overexpression of NLS-ddMisCas13b-SNAPf-NLS-3xFlag in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-ddMisCas13b-SNAPf-NLS-3xFlag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWM_12x601_35bpLinker

    Plasmid

    #157790

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 35 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_35bp linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA

    Plasmid

    #205318

    Purpose

    All-in-one AAV vector expressing ISDge10 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    humanized ISDge10 TnpB
    

    Use

    AAV

    Tags

    3 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid

    #227322

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge