We narrowed to 16,111 results for: form

1. CBSH3+ shRNA-2

   Plasmid

   #160068

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CBSH3+ shRNA-1

   Plasmid

   #160066

   Purpose

   Knock Down SH3+ Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIG1_UL136_26kDa_Myc

   Plasmid

   #74955

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 26kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Strep II Tag (GGGGWSHPQFEKGGGGWSHPQFEK)

   Expression

   Mammalian

   Mutation

   delta (1-62) M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIG1_UL136_33kda_Myc

   Plasmid

   #74957

   Purpose

   Plasmid for mammalian expression of HCMV protein UL136. Expresses only the 33kda isoform of UL136. Also expresses EGFP.

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Insert

   UL136 (UL136 Human Cytomegalovirus strain TB40/E)
   

   Use

   Lentiviral

   Tags

   Myc tag (EQKLISEEDL)

   Expression

   Mammalian

   Mutation

   M63A M78A M80A M100A M128A

   Promoter

   CMV, Lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEBG MKK3b(Glu, delete LXL)

   Plasmid

   #50452

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Tags

   Gst

   Expression

   Mammalian

   Mutation

   deleted L25-I27, changed Serine 218 to Glutamic A…

   Promoter

   SV40

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pGFP-mTet1s pc3901

   Plasmid

   #201700

   Purpose

   Expresses of GFP-tagged mTet1s in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s (Tet1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2xFlag CMV2-YAP2-1st WW mutant

   Plasmid

   #19046

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein isoform 3 (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   Tryptophan 199 changed to Alanine,and Proline 202…

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. p28-2iDP-puro

   Plasmid

   #88893

   Purpose

   Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dual-intron DMD platform
   luciferase
   puromycin resistance enzyme
   mCherry
   

   Expression

   Mammalian

   Mutation

   Truncated version of the dystrophin protein (tran…

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. attB-GFP-Dnmt3b1-Poly(A)

   Plasmid

   #65531

   Purpose

   Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

   Depositor

   Sebastian Bultmann

   Article

   Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

   Insert

   Dnmt3b (Dnmt3b Mouse)
   

   Use

   Mouse Targeting; Bxb1

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   isoform 1

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/TO-mNeonGreen-BRD4

    Plasmid

    #204692

    Purpose

    This plasmid allows for inducible expression of short BRD4 isoform tagged with mNeonGreen, in mammalian cells.

    Depositor

    Michael Rosen

    Article

    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

    Insert

    BRD4 (BRD4 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAX-mScarlet-FBP17

    Plasmid

    #178366

    Purpose

    Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Expression

    Mammalian

    Mutation

    400 Glutamic Acid changed to Aspartic acid (403 A…

    Promoter

    Chicken Beta Actin

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-FCHO1

    Plasmid

    #196910

    Purpose

    visualization of clathrin-coaed vesicle formation in living mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

    Insert

    FCHO1 (FCHO1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. α-catenin ECFP-YPET FRET ΔF-actin binding site

    Plasmid

    #198245

    Purpose

    Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

    Depositor

    Deborah Leckband

    Article

    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

    Insert

    α-catenin ECFP-YPET FRET sensor ΔF-actin binding site
    

    Tags

    ECFP and YPET

    Expression

    Mammalian

    Mutation

    Deleted F-actin binding site on a-catenin (849-90…

    Promoter

    CMV

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. attB-GFP-Dnmt3b6-Poly(A)

    Plasmid

    #65532

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmcherry-mTet1s pc3905

    Plasmid

    #201703

    Purpose

    Expresses of mcherry-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET32a-hEPGN

    Plasmid

    #199232

    Purpose

    Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

    Depositor

    Nilson Zanchin

    Article

    Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

    Insert

    Epithelial mitogen isoform 6 (EPGN Human)
    

    Tags

    TrxA-6xHis-S-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CBSH3- shRNA2

    Plasmid

    #160210

    Purpose

    Knock Down MTLSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat, Human)
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CBSH3- shRNA3

    Plasmid

    #160212

    Purpose

    Knock Down MYDSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat, Human)
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CBSH3- shRNA1

    Plasmid

    #160208

    Purpose

    Knock Down MQWSH3- Collybistin isoforms

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat, Human)
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 c-SRC (WT)

    Plasmid

    #42202

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    c-SRC (SRC Human)
    

    Expression

    Mammalian

    Mutation

    L176F mutation compared to reference sequence NM_…

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDGB3omega1_KanR_BastaR

    Plasmid

    #186426

    Purpose

    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)

    Depositor

    Lukas Fischer

    Article

    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007

    Inserts

    KanR
    BastaR
    

    Use

    Synthetic Biology; Binary vector for escherichia …

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    CaMV 35S and Pnos

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA 3.1 SIRT1-FL

    Plasmid

    #105670

    Purpose

    Mammalian expression construct of mouse SIRT1 full length isoform

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PA-DAD-wt

    Plasmid

    #53095

    Purpose

    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

    Depositor

    Klaus Hahn, Ronen Zaidel-Bar

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    LOV(wt)-6aa-DAD
    

    Tags

    6x His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    L47F and C49S in Venus

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pIE-TGFB2-H6

    Plasmid

    #128500

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (TGFB2 Human)
    

    Tags

    His tag (6x)

    Expression

    Insect

    Promoter

    IE1

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pS6-TGFB2-H8

    Plasmid

    #128502

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (L21-S414) (TGFB2 Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. attB-mCh-Dnmt3b1-Poly(A)-PuroR

    Plasmid

    #65556

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Puromycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. attB-mCh-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65554

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pS6-TGFB2-STREP

    Plasmid

    #128503

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (TGFB2 Human)
    

    Tags

    STREP (2X) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. attB-mCh-Dnmt3b1-Poly(A)-NeoR

    Plasmid

    #65553

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. attB-GFP-Dnmt3b1-Poly(A)-NeoR

    Plasmid

    #65551

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 1

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGFP-mTet1s_K852R pc3914

    Plasmid

    #201704

    Purpose

    Expresses of GFP-tagged mTet1s_K852R in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s-K852R (Tet1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lysine 852 mutated to arginine

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGFP-mTet1s_K852E pc3915

    Plasmid

    #201705

    Purpose

    Expresses of GFP-tagged mTet1s_K852E in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s-K852E (Tet1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Lysine 852 mutated to glutamate

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. CBSH3+ 3m shRNA-1

    Plasmid

    #160067

    Purpose

    3-point mutation negative control for CBSH3+ shRNA-1 targeting collybistin isoforms with SH3+ domain (plasmid 160066)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Mutation

    GGcTGaTTaCAACAAGGATTG (mutations shown in lower c…

    Promoter

    mouse U6 promoter

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. attB-GFP-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65552

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pnCS_SEPT7_SEPT9_i1-TEV-Strep

    Plasmid

    #174500

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i1

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5 CMV 3xFG eIF4G1 +MIC

    Plasmid

    #158776

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pnCS_SEPT7_SEPT9_i5-TEV-Strep

    Plasmid

    #174502

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i5

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208038

    Purpose

    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, N-terminal Split-TurboID

    Promoter

    EFS

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. AAV phSyn1(S)-FlpE-bGHpA

    Plasmid

    #50364

    Purpose

    Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    FlpE recombinase gene
    

    Use

    AAV

    Promoter

    hSyn1

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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43. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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44. pcDNA3.1-Flag-PGC-1alpha2

    Plasmid

    #45502

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform2 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pBoBi-hPEN2 D90A

    Plasmid

    #183641

    Purpose

    Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 (PSENEN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    residues 90 of PEN2 mutated to alanine

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. pBoBi-hPEN2 2A

    Plasmid

    #183640

    Purpose

    Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 (PSENEN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    residues 35 and 40 of PEN2 mutated to alanine

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. TRE-mClover3-TDP-43ΔNLS

    Plasmid

    #214917

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid

    #162877

    Purpose

    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Insert consists of AA1-170

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. pLL3.7-EF-EYFP-YAP1_5SA-PolyA

    Plasmid

    #112285

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) 5SA active mutant - serines 61, 109, 127, 164 and 397 (also known as 381 in other isoforms)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with 5 Serines (61, 109, 127, 1…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid

    #162876

    Purpose

    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy

    Depositor

    Frederic Meunier

    Article

    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

    Insert

    GFP Binding Protein tagged with mEos2
    

    Tags

    mEos2

    Expression

    Mammalian

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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