We narrowed to 1,873 results for: FRT

1. pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

   Plasmid

   #71761

   Purpose

   mGFP and Synaptophysin-mRuby expression from Flp-expressing neurons

   Depositor

   Liqun Luo

   Article

   Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

   Inserts

   mGFP
   Synaptophysin
   

   Use

   AAV

   Tags

   Palmitoylation signal and mRuby

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SP100

   Plasmid

   #187584

   Purpose

   Express FLAG epitope and APEX2-tagged SP100 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SP100 (SP100 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

   Plasmid

   #187587

   Purpose

   Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SMN2 (SMN2 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)

   Plasmid

   #204696

   Purpose

   This plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT(deltaMIN) (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of the amino acids 355-505 of NUT

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

   Plasmid

   #204695

   Purpose

   This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.

   Depositor

   Michael Rosen

   Article

   Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

   Insert

   BRD4-NUT(MIN) (BRD4, NUTM1 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   deletion of majority of NUT, retaining only amino…

   Promoter

   CMV

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

   Plasmid

   #187577

   Purpose

   Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   FBL (FBL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

   Plasmid

   #51248

   Purpose

   Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli3 (Gli3 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV and Flag

   Expression

   Mammalian

   Mutation

   amino acids 849,865,877,907,980,1006 mutated to A…

   Promoter

   EF1a

   Available Since

   March 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

   Plasmid

   #187581

   Purpose

   Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   COILIN (COIL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

   Plasmid

   #187586

   Purpose

   Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5-FRT/TO-3*Flag-APEX2 N-term WRAP53

    Plasmid

    #187578

    Purpose

    Express FLAG epitope and APEX2-tagged WRAP53 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    WRAP53 (WRAP53 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

    Plasmid

    #164131

    Purpose

    Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

    Plasmid

    #114370

    Purpose

    expresses Flpo recombinase-dependent GtACR1

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR1-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

    Plasmid

    #52549

    Purpose

    transgenic construct for expression of GFP and Flpo

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    Flpo recombinase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

    Plasmid

    #114369

    Purpose

    expresses Flpo recombinase-dependent GtACR2

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

    Plasmid

    #197943

    Purpose

    Expression of CoA-Snifit in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

    Insert

    Halo-GFP-ecPanK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PanK=PanK-G41S, D127A,L277W,F252Y

    Promoter

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

    Plasmid

    #60814

    Purpose

    mammalian expression vector

    Depositor

    Daniel Durocher

    Article

    Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Tags

    eGFP-NLS (SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

    Plasmid

    #161577

    Purpose

    Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFRT-TO-RPB1-His K1268R CRres si2,4R

    Plasmid

    #139405

    Purpose

    Dox-inducible, K1268R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

    Depositor

    Jesper Svejstrup

    Article

    Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

    Insert

    RPB1 (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    K1268R

    Promoter

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Syn-FRT-MCS-WPRE-SV40 - GCH9

    Plasmid

    #230819

    Purpose

    AAV cloning vector for Flpo-dependent expression under the Syn promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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