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Showing: 981 - 1000 of 23371 results

  1. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Raul Perez-Jimenez, Benjamin Kleinstiver
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

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  5. pMCRi

    Plasmid
    #207524
    Purpose
    Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR
    Use
    Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207524-plasmid-map-sequence-id-409593

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  6. pNDGG002

    Plasmid
    #231308
    Purpose
    Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Insert
    BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231308-plasmid-map-sequence-id-462187

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  7. TACR1-DuET

    Plasmid
    #213385
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TACR1 (TACR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213385-plasmid-map-sequence-id-430892

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  8. pN3-SP4FL

    Plasmid
    #107719
    Purpose
    Expression plasmid for full-length human Sp4
    Depositor
    Guntram Suske
    Insert
    Sp4 (SP4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 18, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107719-plasmid-map-sequence-id-207621

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  9. pCLIPf-NK1R Control Plasmid

    Plasmid
    #101125
    Purpose
    Expression of CLIP-tagged Neurokinin-1 Receptor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    Neurokinin-1 Receptor, Truncated (TACR1 Human)
    Use
    Tags
    10xHis, CLIP-tag (CLIPf), and FLAG Epitope
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101125-plasmid-map-sequence-id-194114

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  16. pNDGG051

    Plasmid
    #231320
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-pBBR1-Sp-par-ELT4; GG assembly from pOGG004, pNDGG002, pOGG011, pOGG012, and pOGG014, SpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    231320-plasmid-map-sequence-id-463765

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  17. pEditC

    Plasmid
    #207532
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207532-plasmid-map-sequence-id-409609

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  18. pEditA-RY

    Plasmid
    #207533
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→ABE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207533-plasmid-map-sequence-id-409612

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  19. pEditC-RY

    Plasmid
    #207534
    Purpose
    Plasmid for cytidine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    xylS (cured of BsaI-sites), Pm→CBE:SpRY, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207534-plasmid-map-sequence-id-409613

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  20. pNDGG058

    Plasmid
    #231326
    Purpose
    BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RSF1010-Sp-par-ELT4; GG assembly from pGQ0015, pNDGG002, pNDGG005, pOGG012, and pOGG014, SpR
    Depositor
    Barney Geddes
    Article
    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    231326-plasmid-map-sequence-id-471992

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Showing: 981 - 1000 of 23371 results