We narrowed to 2,461 results for: T2A

1. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

   Plasmid

   #130665

   Purpose

   Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
   

   Use

   Ae. aegypti expression

   Expression

   Insect

   Promoter

   Aedes aegypti poly-ubiquitin

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hsyn-DIO-AppNL-G-F-T2A-mCherry

   Plasmid

   #242649

   Purpose

   Floxed (DIO) AAV transgene designed to be packaged in AAV and to express the amyloid precursor protein (App) with familial mutations AppNL-G-F in a Cre-recombinase-dependent manner.

   Depositor

   William Wisden

   Insert

   Floxed (DOI) AAV transgene expressing an amyloid precursor protein with familial mutations and an mCherry tag
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Mutation

   The amyloid precursor reading frame contains a c…

   Promoter

   human synapsin

   Available Since

   Oct. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1α-DIO-cOpn5-T2A-EGFP

   Plasmid

   #237858

   Purpose

   The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

   Depositor

   Minmin Luo

   Article

   Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

   Insert

   Opn5 (OPN5 Chicken)
   

   Use

   AAV and Cre/Lox

   Promoter

   EF1α

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1α-DIO-cOpn5-T2A-mcherry

   Plasmid

   #238009

   Purpose

   The transfer plasmid for packaging AAV expressing Cre-dependent chicken OPN5 was constructed by inserting the OPN5 coding sequence into the multiple cloning site under the control of EF1α promoter

   Depositor

   Minmin Luo

   Article

   Dai et al Sci China Life Sci. 2022 Jul;65(7):1271-

   Insert

   Opn5 (OPN5 Chicken)
   

   Use

   AAV and Cre/Lox

   Promoter

   EF1α

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-1

   Plasmid

   #200539

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-2

   Plasmid

   #200540

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMC-ME_T2A-BFP-P2A-NTRv2-pA ORF-3

   Plasmid

   #200541

   Purpose

   mini circle middle entry plasmid for T2A- BFP- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   BFP, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

   Plasmid

   #200529

   Purpose

   mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   mCherry, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

   Plasmid

   #200530

   Purpose

   mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

   Depositor

   Junsu Kang

   Insert

   mCherry, NTRv2
   

   Use

   Mini-golden

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

    Plasmid

    #200531

    Purpose

    mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

    Depositor

    Junsu Kang

    Insert

    mCherry, NTRv2
    

    Use

    Mini-golden

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. LIG4 HAL-T2A-GFP-SV40pA-HAR

    Plasmid

    #225357

    Purpose

    This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    T2A-GFP-SV40pA
    

    Use

    CRISPR

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. LIG4 HAL-T2A-HYG-SV40pA-HAR

    Plasmid

    #225358

    Purpose

    This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    T2A-HYG-SV40pA
    

    Use

    CRISPR

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. LIG4 HAL-T2A-NEO-SV40pA-HAR

    Plasmid

    #225359

    Purpose

    This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach.

    Depositor

    Sujatha Jagannathan

    Article

    Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

    Insert

    T2A-NEO-SV40pA
    

    Use

    CRISPR

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEtv5-Bsd-T2a-Fkbp-3xF-GGSG

    Plasmid

    #235525

    Purpose

    Etv5 N-term targeting vector, Long Isoform, Blasto-T2A

    Depositor

    Brian Hendrich

    Insert

    Etv5 (Etv5 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Bsdd, FKBP, 3xFLAG

    Mutation

    None

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

    Plasmid

    #238286

    Purpose

    For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRE3G-mCherry-P2A-T2A-nanobody-KS-FtoA

    Plasmid

    #238288

    Purpose

    For integration of mCherry-P2A-T2A-nanobody-KS-FtoA

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2A-T2A-nanobody-KS-FtoA
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

    Plasmid

    #238283

    Purpose

    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4TO F8.2(S54L+T127A)-T2A-mCherry

    Plasmid

    #225586

    Purpose

    Mammalian expression vector for Doxycycline inducible expression of F8.2(S54L+T127A) anti-tau nanobody with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    F8.2(S54L+T127A)-T2A-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV pSYN eGFP T2A SP HALO HAPLN1

    Plasmid

    #228199

    Purpose

    AAV vector expressing GFP and HaloTag-HAPLN1 under synaptophysin promoter

    Depositor

    Sungjin Park

    Article

    Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

    Insert

    HAPLN1 (Hapln1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human synaptophysin promoter

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-hU6-cs1-PuroR-T2A-BFP

    Plasmid

    #230934

    Purpose

    gRNA cloning vector with direct capture sequence 1 in stem loop 2

    Depositor

    Charles Gersbach

    Article

    Liu et al Cell Genom. 2025 Feb 4:100766. doi: 10.1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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