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pEN_TRmiRc2

Plasmid

#25750

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

dsRed2

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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TLD_010

Plasmid

#19696

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pET3a-AqAdk

Plasmid

#18089

Depositor

Dorothee Kern

Article

Henzler-Wildman et al Nature. 2007 Dec 6. 450(7171):838-44.

Insert

adenylate kinase (kad Aquifex aeolicus)

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3_pLyn_EGFP

Plasmid

#214605

Purpose

Expresses fluorescent protein EGFP targeted to plasma membrane

Depositor

Peter Dedecker

Article

Valenta et al Talanta. 2024 Mar

Insert

lyn-EGFP

Tags

lyn targetting

Expression

Mammalian

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Gateway Cloning

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAd5-B3/4

Plasmid

#175785

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B3 and pAd5-B4.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Adenovirus 5 genomic region 10862-18018

Use

Adenoviral and Synthetic Biology

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_4

Plasmid

#130514

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMINTC3GH (Apr)

Plasmid

#110078

Purpose

Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)

Plasmid

#70742

Purpose

1 color weak reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

Plasmid

#70741

Purpose

1 color strong reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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GFP S2TAG

Plasmid

#68296

Purpose

S2TAG GFP reporter

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

GFP S2TAG

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-mCherry-L2

Plasmid

#32416

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

mCherry

Use

Gateway Cloning

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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Histone Deacetylase 5

Plasmid

#21626

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

Histone Deacetylase 5 (Tcrg-C1 Human)

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pCM5.40

Plasmid

#19393

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

glp-1 3'utr in pDONRP2RP3 (glp-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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FKBP-eGFP-TAZ

Plasmid

#221213

Purpose

part A of TAZ-SPARK-OFF

Depositor

Xiaokun Shu

Article

Chung et al Proc Natl Acad Sci U S A. 2024 Feb 13;12

Insert

FKBP-eGFP-TAZ

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_scrambled_gRNA_2

Plasmid

#196628

Purpose

Scrambled control 2 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 2

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pHSP70B-CP6-nptII

Plasmid

#165876

Purpose

Basic G418 resistance cassette in Alpha1, lacks physical marker or attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Basic Selection Vector

Use

Synthetic Biology

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-mChe

Plasmid

#162753

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_84_Citrine

Plasmid

#130584

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_84

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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