165,688 results

1. pCX-DI-2A

   Plasmid

   #74671

   Purpose

   Mammalian expression of DI-2A (hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   De Giorgi et al Nucleosides Nucleotides Nucleic Acids. 2

   Inserts

   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXB1-CfaC-Gyra

   Plasmid

   #122950

   Purpose

   Bacterial expression of C-intein

   Depositor

   Thomas Muir

   Article

   Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

   Insert

   CfaC
   

   Expression

   Bacterial

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-AAV-syn-jGCaMP8f-WPRE

   Plasmid

   #162376

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Q315L

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-miniTurbo

   Plasmid

   #219822

   Purpose

   EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   EF1a

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. bdSUMO-HA2-GFP-HiBit

   Plasmid

   #171017

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-HA2-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWL078 MCP-2xGFP

   Plasmid

   #197047

   Purpose

   pCYC-NLS-MCP-2xGFP on pRS305

   Depositor

   Robert Singer

   Article

   Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

   Insert

   MCP-2xGFP
   

   Expression

   Yeast

   Promoter

   CYC1

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEY11

   Plasmid

   #191017

   Purpose

   Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

   Depositor

   Eviatar Yemini, Oliver Hobert

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   Ultrapanneuronal
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p7SL-neo-TET

   Plasmid

   #51285

   Purpose

   expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   7SL RNA (RN7SL2 Human)
   

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Promoter

   7SL upstream pollII enhancer

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0794

   Plasmid

   #142239

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATF7 (ATF7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC7A9

    Plasmid

    #132245

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC7A9 (SLC7A9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pTXB1-AbaSFI

    Plasmid

    #86900

    Purpose

    Express CBD and intein tag containing AbaSI enzyme full length

    Depositor

    Matthias Bochtler

    Article

    Kazrani et al Nucleic Acids Res. 2014 May;42(9):5929-3

    Insert

    AbaSDFI
    

    Tags

    CBD

    Promoter

    T7

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GAPDH

    Plasmid

    #155622

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    GAPDH (GAPDH Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLentiCas9-T2A-GFP

    Plasmid

    #78548

    Purpose

    Cas9 protein with GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    T2A-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-PIG-empty

    Plasmid

    #105594

    Purpose

    empty control vector with GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    no insert, empty control vector
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    MSCV-LTR

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGH044_mCherry

    Plasmid

    #85412

    Purpose

    lentiviral expression vector for mCherry

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid

    #183831

    Purpose

    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells

    Depositor

    Aaron Hata

    Article

    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

    Inserts

    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    

    Use

    Lentiviral

    Tags

    The fusion protein of EML4 exon 1-6a and ALK exon…

    Expression

    Mammalian

    Mutation

    L1196M/G1202R

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRVV210

    Plasmid

    #87628

    Purpose

    Drives Bxb1 expression in the D. melanogaster germ line.

    Depositor

    Richard Mann

    Article

    Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

    Insert

    Bxb1
    

    Expression

    Insect

    Promoter

    vasa

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-MBP

    Plasmid

    #112568

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-MBP and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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