179,354 results
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Plasmid#162345PurposeLentiviral expression of ASCL1 under the control of the TetON promoterDepositorAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
Plasmid#161927PurposeDonor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSurface marker and Citrine repression reporter
UseSynthetic BiologyExpressionMammalianPromoterpEFAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163029PurposeFLiCRE TF for neuronal expressionDepositorInsertNrxn3b-MKII-hLOV1-TEVcs-tTA
UseAAVExpressionMammalianPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hACE2-hygro
Plasmid#161758PurposeExpresses human ACE2 in mammalian cellsDepositorAvailable sinceNov. 5, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEGBacmam-NGS Wnt-Fc
Plasmid#159629PurposeExpress Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid researchDepositorInsertFzd7/8 subtype NGS Wnt Fc
TagsFc tag and His tagExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Sec61B
Plasmid#141152PurposeFluorescent labeling of ER membrane in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec61 beta (SEC61B Human)
TagsSNAP tagExpressionMammalianMutationL79M on Sec61BPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MAC-NRP1
Plasmid#158384PurposeMAC-tagged gene expressionDepositorAvailable sinceAug. 25, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dCas13-inactive M3nls
Plasmid#157854PurposeTargeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
ExpressionMammalianMutationPspCas13b Δ984-1090 H133A; METTL3 D395AAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-PspCas13b-gRNA-Actb1216
Plasmid#155368PurposePspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPspCas13b gRNA targeting Actb 1216
UseCRISPRExpressionMammalianAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-Tet
Plasmid#138086PurposeEmpty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particlesDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterminimal CMV promoter with heptamerized tet‐operat…Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3XFLAG-VP64-SadCas9-NLS-VP64
Plasmid#135338Purpose3xFLAG 2xVP64 SadCas9 for single guide cloningDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSadCas9
Tags3xFLAG-VP64-SV40 NLS and NLS-VP64ExpressionMammalianMutationD10A and N580APromoterCMVAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol1-UAS:CoChR-tdTomato
Plasmid#124233PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomatoDepositorInsertCoChR-tdTomato
UseZebrafish expressionTagstdTomatoPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sp-sgRNA-mDNMT1_nick-lenti
Plasmid#135959PurposeS. pyogenes nicking sgRNA for mouse DNMT1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmDNMT1_nick
ExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-VprBP
Plasmid#133586PurposeExpresses human VprBP wildtype in mammalian cellsDepositorInsertVpr (HIV-1) binding protein (DCAF1 Human)
TagsHAExpressionMammalianMutationWildtype human VprBP (Dcaf1)Available sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-QUE2m
Plasmid#129350PurposeATP measurementDepositorInsertQUEEN-2m
ExpressionBacterialAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
12xMoonTag-12xSunTag-kif18b-24xPP7
Plasmid#128606PurposeExpresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsExpressionMammalianPromoterPcmvAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-dLight1.3b
Plasmid#125560PurposeFor expression of the genetically encoded dopamine sensor dLight1.3bDepositorHas serviceAAV1, AAV5, and AAV9InsertdLight1.3b
UseAAVTagsFlag tagExpressionMammalianPromoterCAGAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CROPseq-Guide-Zeo
Plasmid#127173PurposeEntry plasmid for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-ymNeongreen-CaURA3
Plasmid#125703PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection. *An updated version of this plasmid is available: #168059 pFA6a-link-ymNeongreen-URA3.DepositorInsertymNeongreen
ExpressionYeastAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by