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179,359 results

Showing: 10101 - 10120 of 179359 results

  2. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Eugene Yeo
    Publication
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74705-plasmid-map-sequence-id-151422

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  6. R777-E285 Hs.ROCK2

    Plasmid
    #70569
    Purpose
    Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK2 (ROCK2 Human)
    Use
    Gateway Cloning
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70569-plasmid-map-sequence-id-126018

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  10. S1PR1-Tango

    Plasmid
    #66496
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S1PR1 (S1PR1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66496-plasmid-map-sequence-id-347878

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  13. pQE-60 roGFP2-His

    Plasmid
    #65046
    Purpose
    Bacterial expression of His-tagged roGFP2
    Depositor
    Tobias Dick
    Insert
    roGFP2
    Tags
    His
    Expression
    Bacterial
    Mutation
    see sequence
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65046-plasmid-map-sequence-id-350692

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  14. pGEM5Z(+)-EGFP

    Plasmid
    #65206
    Purpose
    EGFP expression plasmid (also called p111) for the creation of a heteroduplex DNA repair assay plasmid with p189. *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Publication
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Restriction enzyme recognition sites added either…
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65206-plasmid-map-sequence-id-490956

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  16. pFRT-TODestFLAGHA_RNAseH1

    Plasmid
    #65782
    Purpose
    expresses FLAGHA-tagged RNAsH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Human, Zebrafish)
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65782-plasmid-map-sequence-id-345165

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  17. pCAG-HA-Nrxn1beta AS4(+)

    Plasmid
    #59410
    Purpose
    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]
    Depositor
    Peter Scheiffele
    Publication
    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nrxn1beta AS4(+) (Nrxn1 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59410-plasmid-map-sequence-id-107054

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  18. Ai62(TITL-tdT) Flp-in replacement vector

    Plasmid
    #61576
    Purpose
    Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus
    Depositor
    Hongkui Zeng
    Publication
    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10
    Insert
    chromatin insulator flanked TRE-LSL-tdTomato
    Use
    Recombinase-mediated cassette exchange using flp …
    Available since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61576-plasmid-map-sequence-id-209910

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  19. pSBbi-RN

    Plasmid
    #60519
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene
    Depositor
    Eric Kowarz
    Publication
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60519-plasmid-map-sequence-id-97527

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Showing: 10101 - 10120 of 179359 results