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Showing: 10121 - 10140 of 17309 results

  1. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

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  2. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  3. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  4. pEG608

    Plasmid
    #40194
    Depositor
    Geraldine Seydoux
    Article
    Griffin et al Cell. 2011 Sep 16;146(6):955-68.
    Insert
    Mex-5 (RNAi resistant) (mex-5 Nematode)
    Tags
    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…
    Expression
    Worm
    Mutation
    Exon 2 recoded to be RNAi-resistant
    Promoter
    4.4 kb mex-5 promoter fragment
    Available Since
    Sept. 19, 2012
    Availability
    Academic Institutions and Nonprofits only

  5. pLEXm-hCD45d

    Plasmid
    #247052
    Purpose
    For mammalian expression of human CD45 domains 1 and 2 with C-terminal Maltose-Binding Protein fusion for secretion into the medium
    Depositor
    Omer Dushek
    Article
    Vankayala et al Bioconjug Chem. 2026 Feb 18;37(2):281-29
    Insert
    hCD45d1d2-MBP
    Tags
    His6 and Maltose binding protein (MBP)
    Expression
    Mammalian
    Mutation
    Domains 1 and 2 only, corresponding to residues 2…
    Promoter
    chick β-actin promoter
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    247052-plasmid-map-sequence-id-492518

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  6. pAL950

    Plasmid
    #119748
    Purpose
    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Tags
    none
    Expression
    Bacterial
    Mutation
    Internal NdeI site removed and mutations Alanine …
    Promoter
    T7
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119748-plasmid-map-sequence-id-232481

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  7. piggyBac_rtTA (4th_Gen)_SNCA-Avi_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1628)

    Plasmid
    #209086
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(Avi tagged) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Tags
    AviTag
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available Since
    Dec. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    209086-plasmid-map-sequence-id-417159

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  8. piggyBac_rtTA (4th_Gen)_SNCA_IRES_NGN2-2A-PURO_Synapsin-BirA (pEHA1623)

    Plasmid
    #209085
    Purpose
    Ngn2 mediated cortical neuron induction, SNCA(wt) expression, Synapsin promoter BirA ligase expression
    Depositor
    Vikram Khurana
    Article
    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.
    Inserts
    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    BirA
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    TRE4G and hSyn
    Available Since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    209085-plasmid-map-sequence-id-416546

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  10. p1.1-mCherry

    Plasmid
    #162772
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    red fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162772-plasmid-map-sequence-id-339102

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  11. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

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  12. Venus-Bad-4E-pEGFP-C1

    Plasmid
    #166740
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1
    Insert
    Bad-4E (BAD Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166740-plasmid-map-sequence-id-329000

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  13. pelB-MBP-HtrA-ss

    Plasmid
    #137066
    Purpose
    E. coli expression clone (T7lac promoter) for mature HtrA (aa 28-474) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature HtrA: lacks the HtrA signal peptide
    Promoter
    T7lac
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    137066-plasmid-map-sequence-id-291956

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  17. pGBW-m4046848

    Plasmid
    #149257
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149257-plasmid-map-sequence-id-297009

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  18. pGBW-m4046906

    Plasmid
    #149248
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp2
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149248-plasmid-map-sequence-id-357572

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  19. pGBW-m4046793

    Plasmid
    #145758
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145758-plasmid-map-sequence-id-298376

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  20. pGBW-m4046966

    Plasmid
    #145733
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    WT, AA 2-289
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145733-plasmid-map-sequence-id-323977

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Showing: 10121 - 10140 of 17309 results