We narrowed to 46,132 results for: cha

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CBEd SpG UGI Puro

Plasmid

#235045

Purpose

Cytosine base editor d with SpG nicking Cas9 makes C > T edits

Depositor

Justin Pritchard

Article

Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

CBEd, nCas9

Use

CRISPR and Lentiviral

Tags

P2A-PuroR

Promoter

EF1a core

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 N-RAS

Plasmid

#236450

Purpose

transient overexpression of N-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

N-RAS (NRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-MCS--4x6T

Plasmid

#196420

Purpose

Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV and Cre/Lox; Astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pHJ4: LexA-VHH-Caffeine

Plasmid

#108242

Purpose

LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

Depositor

Jerome Bonnet

Article

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

LexA DNA binding domain fusing with VHH-Caffeine antibody

Use

Synthetic Biology

Promoter

pLacO1

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-TTc-4xDQNAT

Plasmid

#128401

Purpose

Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Promoter

trc

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-29b-1/miR-29a

Plasmid

#64231

Purpose

Expresses miR-29b-1 and miR-29a in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-29b-1 and miR-29a (MIR29A, MIR29B1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pMax_mOrange_G299C

Plasmid

#177829

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_G299C

Expression

Mammalian

Mutation

G299C

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pPPED

Plasmid

#162468

Purpose

Destination Expression vector for dicot plant prime editing

Depositor

Adam Bogdanove

Article

Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

Inserts

Cas9H840A
attR gateway destination cassette

Tags

MLV reverse transcriptase

Expression

Plant

Mutation

Change histidine 840 to Alanine based sequence o…

Promoter

2x35S

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluu

Plasmid

#106122

Purpose

Fast indicator for synaptic glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR S72T variant

Tags

Myc and PDGFR

Expression

Mammalian

Mutation

Changed Ser 72 to Thr

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

Plasmid

#227614

Purpose

Overexpression of SLC25A46 in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Available Since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

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UAST-FRT-GFP-FRT-uMCas12a+nls1x

Plasmid

#230908

Purpose

Double inducible expression of Cas12a+ with Gal4 and Flp.

Depositor

Michael Boutros

Article

Port et al Nat Commun. 2026 Jan 15;17(1):877. doi:

Insert

Cas12a+

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-trGluc

Plasmid

#158959

Purpose

DNA donor plasmid containing repair template for Gaussia luciferase (Gluc) of bioluminescent repair reporter (BLRR).

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

trGluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-HLA-G SCT-T2A-GFP

Plasmid

#205462

Purpose

lentiviral plasmid for expression of HLA-G SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

HLA-G SCT (HLA-G Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGal4AD-CIB1

Plasmid

#28245

Purpose

Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

Gal4AD (CIB1, GAL4 Budding Yeast)

Tags

CIB1

Expression

Yeast

Promoter

ADH1

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGMC00028 (aka pTS0057)

Plasmid

#200832

Purpose

AAVS1 guide RNA cloned into the lentiSAM puro backbone

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Insert

AAVS1 guide RNA

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pclbw-PAGFPomp25

Plasmid

#58427

Purpose

Mammalian expression of photoactivatable GFP targeted to the mitochondrial outer membrane

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

paGFP-omp25 (Synj2bp Mouse)

Use

Retroviral

Tags

PAGFP

Expression

Mammalian

Mutation

contains aa's 109-145 of mouse omp25

Promoter

cmv-bactin

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn_CapChR2_eYFP

Plasmid

#188035

Purpose

Expresses CapChR2-eYFP in mammalian neurons

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR2-eYFP

Use

AAV

Tags

eYFP

Mutation

S43D, L112C, T139C and N238E mutations in CoChR

Promoter

hSyn

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(H840A)-PolI5M

Plasmid

#249064

Purpose

Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9(H840A)-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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