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Endosome-targeted optodPLD

Plasmid

#140055

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMN-321

Plasmid

#195449

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - minCMV - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAGT5439

Plasmid

#192351

Purpose

L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

TTCG_Stop-tOCS_CGCT

Expression

Plant

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTX1-RNA0

Plasmid

#126040

Purpose

In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

RNA0

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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Double UP sfGFP to mScarlet

Plasmid

#120261

Purpose

Natively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

sfGFP/mScarletP2A-mcs

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP-Hygro

Plasmid

#14686

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP and PGK-Hygro

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pMT Draper-Ala11-GFP

Plasmid

#126654

Purpose

Inducible expression of Draper-Ala11-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper cytoplasmi Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic Tyr residues to Ala

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Par6 S345A

Plasmid

#11749

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

PAR6 (Pard6a Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S345A

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
pZS220

Plasmid

#177642

Purpose

In vitro transcription of Patiria miniata Dishevelled

Depositor

Iain Cheeseman

Article

Swartz et al Curr Biol. 2021 Dec 20;31(24):5633-5641.

Insert

Dishevelled

Use

In vitro transcription

Tags

GFP

Mutation

K47A

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pB6N_RB38-Mt-tk_v8_AGKS

Plasmid

#191514

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAN, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE RB38-Mt-tk AGKS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pB7N_LT51-Mt-tk_v8_AGKS

Plasmid

#191515

Purpose

Expression of mitochondrial v8 ZF-DdCBE in mammalian cells (N-terminal DddAC, Left)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

v8 ZF-DdCBE LT51-Mt-tk AGKS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pQCXIZ CMV/TO DEST (w321-2)

Plasmid

#17401

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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258/-2.5CAT

Plasmid

#25431

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter/Enhancer (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Available Since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pMN-294

Plasmid

#195453

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF1 BS - ybTATA - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Expression

Yeast

Promoter

GPD

Available Since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:GFP-T35S

Plasmid

#211836

Purpose

Plant transient expression cassette for GFP (control) under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

GFP control

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pYC46

Plasmid

#63904

Purpose

Recipient integrative vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pQCXIB CMV/TO LUC (w431-1)

Plasmid

#17475

Purpose

Retroviral Luciferase Expression vector, CMV/TO promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Luciferase and Retroviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AKAP1-mCherry-LOVPLD

Plasmid

#219558

Purpose

mitochondria-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

AKAP1-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

July 11, 2024

Availability

Academic Institutions and Nonprofits only

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3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Expression

Bacterial

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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