We narrowed to 9,242 results for: ef1

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pEF1a-myc-LRH1(ES)-IRES-neo

Plasmid

#28092

Depositor

Austin Cooney

Article

Wagner et al Stem Cells. 2010 Oct . 28(10):1794-804.

Insert

Lrh-1 (Nr5a2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pCDF1-MCS2-EF1-Puro-RGS7

Plasmid

#113728

Purpose

Express RGS7 in mamalian cells

Depositor

Yardena Samuels

Article

Qutob et al Sci Rep. 2018 Jan 12;8(1):653. doi: 10.1

Insert

RGS7 (RGS7 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-CHMP3(155)-HA

Plasmid

#154173

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-owl-monkey-retroCHMP3(148)-HA

Plasmid

#154252

Purpose

expresses prematurely truncated owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of retroCHMP3 at amino acid…

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6(BsmBI)-EF1a-BFP-Nkx2-5

Plasmid

#117135

Purpose

Expression vector for Nkx2-5 and gRNA

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

CRISPR and Lentiviral

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]

Plasmid

#128588

Purpose

AAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

EF1α (1.1 kb short version)

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-KIF5A-HA-IRES-Puro

Plasmid

#166952

Purpose

Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Use

Lentiviral

Tags

HA

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti

Plasmid

#138318

Purpose

Lenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFP

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SauCas9 GFP sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV1-EF1α-F-Flex-jGCaMP7b-WPR

Plasmid

#171689

Purpose

Flp-dependent expression of jGCaMP7b

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Bauer et al Neuron. 2021 Aug 4;109(15):2457-2468.e12

Insert

GCaMP7b

Use

AAV

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

Plasmid

#166412

Purpose

express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 K90E (POT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

K90E

Promoter

EF-1a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA

Plasmid

#121806

Purpose

pMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETa

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

HA epitope tag-polyA + EF1a::TETa-polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-FLEX-rc [SomArchon-GFP]

Plasmid

#153532

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter, in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN

Plasmid

#69586

Purpose

Expresses mutated p53-L344P tagged with mKate2 and split N-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53-L344P (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - N term

Expression

Mammalian

Mutation

p53 L344P mutation that prevents dimerization and…

Promoter

EF1alpha

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1-a_LbuCas13a-HA-IRES-BFP-2A-BSD

Plasmid

#251219

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven LbuCas13a expressing cells.

Depositor

Niels Geijsen

Insert

LbuCas13a

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EF-1a

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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p416TEF1 Su9-Cystector (Cysteine biosensor)

Plasmid

#250655

Purpose

Mitochondrial matrix expression plasmid for Cystector (Cysteine biosensor) in Saccharomyces cerevisiae

Depositor

Peter Dedecker

Article

Pedre et al bioRxiv 2026.01.27.698081

Insert

Su9-Cystector (Cysteine biosensor)

Tags

Mitochondrial targetting sequence based on Neuros…

Expression

Yeast

Promoter

TEF1

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV- EF1α core -hKCNJ2-HA tag

Plasmid

#246995

Purpose

Can be used to generate AAV virus that will express human KCNJ2 with Ala115 HA tag from EF1α core promoter

Depositor

Ting Chen

Article

Chen et al Cell. 2025 Aug 14:S0092-8674(25)00857-8.

Insert

hKCNJ2 (KCNJ2 Human)

Use

AAV

Tags

HA tag (Internal at Ala115)

Promoter

EF1α

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2730 - pHR-EF1A-IRES-GFP

Plasmid

#250262

Purpose

Empty vector (GFP only) control.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-RPA194-E593Q

Plasmid

#247349

Purpose

Expresses HaloTag-RPA194-E593Q

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

RPA194 (POLR1A), mutated with E593Q

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#242162

Purpose

Lentiviral expression of dCas9-VPR with puromycin reporter

Depositor

Amy Brock

Insert

dCas9-VP64-RelA-Rta; T2A-puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-50aa-puro

Plasmid

#248153

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 50 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa645 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-16aa-puro

Plasmid

#248152

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 16 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa611 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-3xFLAG-puro

Plasmid

#248155

Purpose

Lentiviral vector expressing human FCRL3 with 3xFLAG tags

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Tags

3x FLAG

Mutation

A 3xFLAG epitope tag is fused in frame to the C-t…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-93aa-puro

Plasmid

#248154

Purpose

Lentiviral vector encoding a truncated form of human FCRL3, containing only the first 93 residues of the cytoplasmic tail.

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Mutation

Mutagenesis to insert a stop codon at aa688 in FC…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1_-FCRL3-IRES-puro

Plasmid

#248148

Purpose

Lentiviral vector encoding human FCRL3

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-fDIO-mCherry-LATeNT

Plasmid

#240991

Purpose

Flp-dependent expression of LATeNT

Depositor

Alice Ting

Article

Roh et al bioRxiv 2025.01.27.635161; doi: 10.1101/

Insert

mCherry-V5-LATeNT

Use

AAV

Tags

V5 (internal)

Expression

Mammalian

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_G198R

Plasmid

#242465

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The G198R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

G198R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_L199R

Plasmid

#242466

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199R mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199R

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_L199G

Plasmid

#242467

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The L199G mutation disrupts XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L199G

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-3XFLAG-hASB8_ANK3-4

Plasmid

#242468

Purpose

Expresses human ASB8 protein with an N-terminal 3xFLAG tag along with a puromycin selection marker. The ANK3-4 mutations disrupt XPO1 binding.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

ANK3-4 = R87A - E95A - K96A - W124G - K128G

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-puro-EF1A-Tag100-hASB8

Plasmid

#242460

Purpose

Expresses human ASB8 protein with an N-terminal Tag100 along with a puromycin selection marker.

Depositor

Dirk Daelemans

Article

Wing et al Nat Chem Biol. 2025 Dec;21(12):2002-2013

Insert

ASB8 (ASB8 Human)

Use

Lentiviral

Tags

Tag100

Expression

Mammalian

Promoter

EF1A

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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