We narrowed to 2,572 results for: GEM

Showing: 2001 - 2040 of 2572 results

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pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII

Plasmid

#80863

Purpose

Phagemid (encodes evolved cI4A5T6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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tFucci(CA)5pPBbsr2

Plasmid

#197351

Purpose

Encoding tFucci(CA)5.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

tFucci(CA)5

Tags

AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-T7RNAP

Plasmid

#74096

Purpose

T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7 RNAP

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx162-Hyg

Plasmid

#210215

Purpose

Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

162xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLEW100cre-EP1

Plasmid

#33345

Purpose

For expression of tetracycline-inducible CRE in Trypanosoma brucei

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Cre/Lox; Trypanosoma brucei expression vector

Promoter

TET operator

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pISH-beta2-microglobulin

Plasmid

#15883

Depositor

Peter Mombaerts

Article

Ishii et al Curr Biol. 2003 Mar 4. 13(5):394-400.

Insert

beta2-microglobulin (B2m Mouse)

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only
pISH-Msx2

Plasmid

#84314

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx2 (Msx2 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSFVCs-lacZ

Plasmid

#92076

Purpose

This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

Depositor

Henrik Garoff

Article

Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

Insert

C-lacZ

Use

C-lacz expression plasmid, for run-off transcript…

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRB

Plasmid

#104475

Purpose

Plasmid carrying FRB with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRB (MTOR Human)

Use

Phagemid

Tags

HA tag

Mutation

includes only the FRB domain

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Expression

Mammalian

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLyn-FRB-mCherry-nmd3-BSD

Plasmid

#85796

Purpose

Expression of PPM mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PPM Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Promoter

Pfnmd3

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cIopt-J23106-geneIII

Plasmid

#80852

Purpose

Phagemid (encodes M13 gene III and lambda cIopt).

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
Lambda cIopt

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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DT037A

Plasmid

#159398

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23101-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

J23101 (strong) promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pSLI-TGD(1463000)

Plasmid

#85791

Purpose

P. falciparum integration plasmid to disrupt genes using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, GFP-2TA-Neo-R

Use

P. falciparum genomic integration (gene disruptio…

Tags

GFP

Promoter

none

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSkipFlox

Plasmid

#85797

Purpose

P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

split Cre recombinase

Use

Cre/Lox; P. falciparum expression

Tags

FKBP, FRB*

Promoter

Pfcrt

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pBP-lldR

Plasmid

#74095

Purpose

L-lactate repressor (LldR) from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

lldR

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTC150

Plasmid

#70014

Purpose

'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Donor + GVR

Expression

Plant

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pPLP1.5

Plasmid

#22614

Depositor

Lynn Hudson

Article

Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

Insert

human Proteolipid Protein cDNA (PLP1 Human)

Use

E. coli phagemid vector

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pLITMUS-rpoN-cI-J23106-geneIII

Plasmid

#80843

Purpose

Phagemid (encodes M13 gene III and lambda cIopt).

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
Lambda cI

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p1xNLS-FRB-mCherry-hsp86-BSD

Plasmid

#85795

Purpose

Expression of 1xNLS mislocaliser in P. falciparum

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

1xNLS Mislocaliser

Use

P. falciparum expression

Tags

FRB*, mCherry

Promoter

Pfhsp86

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTU1-A-RFP

Plasmid

#72939

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Th

Plasmid

#105988

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

tyrosine hydroxylase (Th Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMB1-A-pBAD-dCas9

Plasmid

#190132

Purpose

Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pTH-MYCN

Plasmid

#35416

Depositor

William A. Weiss

Article

Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (MYCN Human)

Expression

Mammalian

Promoter

Tryosine Hydroxlyase (TH)

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-dCas9

Plasmid

#190129

Purpose

Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBP-SJM901

Plasmid

#72966

Purpose

Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

SJM901 promoter

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Article

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Available Since

June 18, 2013

Availability

Academic Institutions and Nonprofits only
pLITMUS-rpoN-cI5C6A,P-J23106-geneIII

Plasmid

#80860

Purpose

Phagemid (encodes evolved cI5C6A,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A,P

Expression

Bacterial

Promoter

BBa_J23106 and rpoN

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Tshz1

Plasmid

#74360

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 1 (Tshz1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Galpha-i2

Plasmid

#15929

Depositor

Peter Mombaerts

Article

Rodriguez et al Nat Neurosci. 2002 Feb . 5(2):134-40.

Insert

Galpha-i2 (Gnai2 Mouse)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only
pTU2-a-RFP

Plasmid

#72958

Purpose

Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - a site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Satb1

Plasmid

#74357

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Satb1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

special AT-rich sequence binding protein 1 (Satb1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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KFS2MT

Plasmid

#44624

Depositor

Robert Oshima

Article

Cecena et al Am J Pathol. 2006 Jan;168(1):310-20.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

polyomavirus middle T antigen (PyMT)

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBP-TL5

Plasmid

#72989

Purpose

RBS_M2

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

M2 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pBP-TL11

Plasmid

#72995

Purpose

RBS_L4

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L4 RBS

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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We narrowed to 2,572 results for: GEM