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pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam E2F6

Plasmid

#37965

Depositor

Jacqueline Lees

Article

Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

Insert

E2F6 (E2F6 Human)

Expression

Mammalian

Available Since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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7xE-Box::Renilla

Plasmid

#124532

Purpose

c-Myc Renilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

c-Myc Renilla reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-2200

Plasmid

#31062

Depositor

Gerhart Ryffel

Article

Thomas et al Hum Mol Genet. 2001 Sep 15. 10(19):2089-

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-2200 to -1 of P2 HNF4A promoter

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGL4.10-Claudin-1

Plasmid

#46387

Purpose

Claudin-1 promoter reporter assay

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

CLDN1 Promoter (CLDN1 Human)

Use

Luciferase

Promoter

CLDN1 promoter

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
PKC epsilon CAT

Plasmid

#21242

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC epsilon (Prkce Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-394

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only
Stat1 alpha Y701F pRc/CMV

Plasmid

#8701

Depositor

Jim Darnell

Article

Wen et al Cell 1995 Jul 28;82(2):241-50.

Insert

STAT1 alpha Y701F (STAT1 Human)

Expression

Mammalian

Mutation

Y701F

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.Flag.HERC2-HECT-WT.6xHis

Plasmid

#39227

Depositor

Aziz Sancar

Article

Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

Insert

HERC2 HECT Domain (HERC2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

T7

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag mPTIP

Plasmid

#15557

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pAd-Track Flag GCN5 Y621A/P622A

Plasmid

#14425

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 Y621A/P622A (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

Y621A, P622A. Catalytically inactive acetyltransf…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP

Plasmid

#119903

Purpose

Bxb1 integration plasmid for cuO array with Puro selection cassette

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

cuOx144 array

Use

Mouse Targeting

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET30a-HS-Tn5

Plasmid

#127916

Purpose

Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Hyperstable Tn5

Tags

E coli elongation factor Ts and Mxe intein - Chit…

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-HNRNPR

Plasmid

#38067

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPR heterogeneous nuclear ribonucleoprotein R (HNRNPR Human)

Tags

FLAG, HA, and His

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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SP6-sgRNA-scaffold

Plasmid

#47912

Purpose

Scaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injection

Depositor

Paul Sternberg

Article

Chiu et al Genetics. 2013 Aug 26.

Type

Empty backbone

Use

CRISPR

Tags

sgRNA 3' end

Expression

Worm

Promoter

SP6

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pET28b::mAID

Plasmid

#15923

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

AID (Aicda Mouse)

Tags

His

Expression

Bacterial

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pMB66

Plasmid

#47946

Purpose

Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

Cas9

Use

CRISPR

Tags

3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

Expression

Worm

Promoter

hsp-16.48

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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