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pUDE1084

Plasmid

#168500

Purpose

expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

Depositor

Jean-Marc Daran

Article

Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

Inserts

Fncas12a
T7RNA polymerase

Use

CRISPR

Promoter

TDH3 and TEF1

Available Since

Dec. 16, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-D53A-GFP

Plasmid

#208778

Purpose

MSCV vector expressing D53A mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Mutation

Changed aspartic acid 53 to alanine (D53A)

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTZ tRNA_gRNA empty scaffold

Plasmid

#188450

Purpose

Expression of gRNAs using a human tRNA promoter

Depositor

Ajit Chande

Article

Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

tRNA glutamine

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBXNH3LCA_TM287/288

Plasmid

#132701

Purpose

expresses TM287/288 in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

TM287/288 (TM0288, TM0287 Thermotoga maritima)

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.HA.HA.Mm.RAP1

Plasmid

#184642

Purpose

Retroviral expression of mouse RAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

RAP1

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

ADE3p

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_+1D

Plasmid

#149389

Purpose

P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

1bp inserted downstream of the IHFprox site

Promoter

pBAD

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV2-Brd4 R1337P

Plasmid

#21939

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, R1337P

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 S1351R

Plasmid

#21941

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa1209-1362 only, S1351R

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFlag-CMV2-Brd4 D1346N

Plasmid

#21940

Depositor

Eric Verdin

Article

Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1209-1362 only, D1346N

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only
pFL-TK-AID3'UTR

Plasmid

#19986

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

Plasmid

#72233

Purpose

Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Expression

Yeast

Promoter

MET25

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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M71::Myc6-IRES-taulLacZ LNL TV

Plasmid

#15619

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::Myc6-IRES-taulLacZ LNL TV (Myc Mouse)

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-C+F-GFP

Plasmid

#209699

Purpose

Expresses GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

GFP

Use

AAV

Promoter

CBA

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM_Δflv3.sm

Plasmid

#185533

Purpose

a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

aadA gene flanked by flv3 upstream and downstream regions

Use

Synthetic Biology

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-C+F-ArchT-GFP

Plasmid

#209700

Purpose

Expresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinases

Depositor

Peer Wulff

Article

Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

Insert

ArchT-GFP

Use

AAV

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPR2_IRdist_scram

Plasmid

#162319

Purpose

P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Insert

Pseudomonas aeruginosa PA14 CRISPR2 locus

Expression

Bacterial

Mutation

The distal Upstream Motif site is scrambled

Promoter

pBAD

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.H10L

Plasmid

#198683

Purpose

An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae

Depositor

Paola Branduardi

Article

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

XI1_UP

Use

Synthetic Biology

Expression

Yeast

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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