We narrowed to 11,788 results for: CHL
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Plasmid#98592PurposeProduces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.DepositorInsertFnCpf1
UseCRISPRExpressionBacterialMutationCodon optimized for E. coliPromoteraraBADAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB541
Plasmid#74875PurposeGateway cloning compatible binary vector for C-terminal fusion with eYFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-N346A/C348A
Plasmid#127411PurposeDouble mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethersDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialMutationN346A-C348APromoteraraBADAvailable SinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE347
Plasmid#153228PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB435
Plasmid#74825PurposeGateway cloning compatible binary vector for C-terminal fusion with LUC (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE334
Plasmid#153243PurposeM2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCas9cur
Plasmid#71538PurposeConstitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.DepositorInsertsCas9
Plasmid curing system
UseCRISPRExpressionBacterialPromoterpBADAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Hygro
Plasmid#25737Purpose3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceAug. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBP-split-intein-Gal4[1-20]-N-int
Plasmid#199201PurposeGateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expressionDepositorInsertsplit-intein-Gal4[1-20]-N-int
ExpressionInsectAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYSD080
Plasmid#212917PurposeEncodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA1 (AGA1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD083
Plasmid#212920PurposeEncodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertSED1 (SED1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD003
Plasmid#212900PurposeEncodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertECM14 (ECM14 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD023
Plasmid#212909PurposeEncodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertMFalpha1 (MF(ALPHA)1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD081
Plasmid#212918PurposeEncodes the HA-tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertAGA2 (AGA2 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD005
Plasmid#212902PurposeEncodes S. cerevisiae KSH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsertKSH1 (KSH1 Budding Yeast)
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-Zeo
Plasmid#25736PurposeLentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Zeocin resistance gene coexpression.DepositorTypeEmpty backboneUseLentiviral and RNAiExpressionMammalianAvailable SinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-hChR2(H134R)-EYFP-WPRE
Plasmid#20942Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralExpressionMammalianMutationH134R mutation in ChR2PromoterEF1alphaAvailable SinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneTagsHis8-SumoExpressionBacterialPromoterT7Available SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCph8
Plasmid#22869DepositorInsertpCph8
UseSynthetic BiologyExpressionBacterialMutationFirst 517 amino acids of Cph1 and the last 229 am…Available SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-1
Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only