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Showing: 10241 - 10260 of 158104 results

  1. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP2 (USP2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pGEMHE-NLS-mEGFP

    Plasmid
    #105527
    Purpose
    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal
    Depositor
    Melina Schuh
    Article
    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31
    Insert
    Nucleoplasmin
    Use
    In vitro transcription
    Tags
    mEGFP
    Expression
    Mutation
    NLS of nucleoplasmin (encoding amino acids KRPAAT…
    Promoter
    T7
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    105527-plasmid-map-sequence-id-201844

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  10. LSB-hsa-miR-218-5p

    Plasmid
    #103357
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-218-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-218-5p target
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103357-plasmid-map-sequence-id-195763

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  13. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

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  16. mCherry-WASH1-C-18

    Plasmid
    #55162
    Purpose
    Localization: Cytoskeleton, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    WASH1
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid
    #20297
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available since
    June 23, 2009
    Availability
    Academic Institutions and Nonprofits only
    20297-plasmid-map-sequence-id-354478

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Showing: 10241 - 10260 of 158104 results