165,688 results

1. pQE31-rsEGFP2

   Plasmid

   #102879

   Purpose

   Expresses rsEGFP2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

   Insert

   rsEGFP2
   

   Tags

   6xHisTag

   Expression

   Bacterial

   Available Since

   Jan. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0675

   Plasmid

   #141659

   Purpose

   Lentiviral vector for overexpressing the PRDM16 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM16 (PRDM16 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHM131

   Plasmid

   #225530

   Purpose

   Encodes destablized mCherry under IBRE4 promoter, and constitutive BFP

   Depositor

   Jared Toettcher

   Article

   McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

   Inserts

   mCherry
   BFP
   

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AbVec2.0-IGHG4

   Plasmid

   #99578

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype

   Depositor

   Hedda Wardemann

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. HsTPC2-EYFP

   Plasmid

   #135194

   Purpose

   Expresses tagged TPC2 in mammalian cells

   Depositor

   Antony Galione

   Article

   Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

   Insert

   TPC2 (TPCN2 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. zWnt5b

   Plasmid

   #21282

   Depositor

   Randall Moon

   Article

   Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

   Insert

   Wnt5b (wnt5b Zebrafish)
   

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. TALED_Left-ND1-1397C-AD

   Plasmid

   #183892

   Purpose

   Expresses TALED_Left-ND1-1397C-AD in mammalian cells

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   TALED for ND1 mutation
   

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-GFP-Syntaxin1A

   Plasmid

   #170871

   Purpose

   Mammalian expression of His-GFP-Syntaxin1A.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Syntaxin 1A (Stx1a Mouse)
   

   Tags

   (His)6 and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-HIS-Tol2

   Plasmid

   #112486

   Purpose

   functional recombinant Tol2 protein from E.coli

   Depositor

   Stephen Ekker

   Article

   Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

   Insert

   Tol2 Transposase (tpase Synthetic)
   

   Tags

   HIS/6x

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ADORA1-Tango

    Plasmid

    #66209

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADORA1 (ADORA1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pWM_12x601_20bpLinker

    Plasmid

    #157787

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with 20 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_20bp linker
    

    Expression

    Bacterial

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. 7TGC

    Plasmid

    #24304

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-mCherry
    

    Use

    Lentiviral

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5155

    Plasmid

    #175391

    Purpose

    Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

    Depositor

    Michael Wiles

    Article

    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting; Cloning vector, to be used to de…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe puro IRES-EGFP

    Plasmid

    #14430

    Depositor

    L. Miguel Martins

    Has Service

    Cloning Grade DNA

    Insert

    IRES-EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCVpuro-Cas9-EGFP

    Plasmid

    #159126

    Purpose

    Expresses active Cas9 fused with EGFP for fluorescence microscopy

    Depositor

    Taekjip Ha

    Article

    Liu et al Science. 2020 Jun 12;368(6496):1265-1269

    Insert

    Cas9 fused to EGFP
    

    Use

    CRISPR and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MSCV LTR promoter

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-scFv-GCN4-mStayGold-GB1-dWPRE

    Plasmid

    #231598

    Purpose

    Express scFv_GCN4-mStayGold for SunTag translation imaging

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    scFv-GCN4-mStayGold
    

    Use

    Lentiviral

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PKC eta DN

    Plasmid

    #21246

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC eta (Prkch Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K384R

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

19. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pscALPS- SIV-MAC251 vpx

    Plasmid

    #115810

    Purpose

    Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVMAC251 Vpx
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Promoter

    SFFV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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