We narrowed to 14,068 results for: crispr grnas

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Blat-BSD

Plasmid

#163791

Purpose

The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAIO-AsCpf1

Plasmid

#137846

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1-NLS-FLAG

Use

CRISPR

Available Since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g1_CASH-1

Plasmid

#188975

Purpose

A human codon-optimized SpCas9 nickase and chimeric g1 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g1 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)_g2_CASH-1

Plasmid

#188976

Purpose

A human codon-optimized SpCas9 nickase and chimeric g2 guide RNA expression plasmid.

Depositor

Sangdun Choi

Article

Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

Insert

g2 guide RNA

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8

Plasmid

#176184

Purpose

gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8_28bp

Plasmid

#176185

Purpose

plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g3 Cas9-T2A-mCherry

Plasmid

#164252

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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sg1

Plasmid

#113966

Purpose

Single short guide RNA targeting GTATAGCATACATTATACG

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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PV521

Plasmid

#132346

Purpose

Cas9 R promoter targeting sgRNA1 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA1

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV523

Plasmid

#132347

Purpose

Cas9 R promoter targeting sgRNA2 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA2

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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PV528

Plasmid

#132348

Purpose

Cas9 R promoter targeting sgRNA3 expression cassette for Zea mays

Depositor

Joshua Young

Article

Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

Insert

Cas9 R promoter targeting sgRNA3

Expression

Plant

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSCIP-mB2M

Plasmid

#154091

Purpose

Self-Cutting and Integrating CRISPR backbone targeting murine B2M

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting murine B2M locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-AAVS1-sg

Plasmid

#194721

Purpose

px458 with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

Cas9 (AAVS1 Streptococcus pyogenes)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJL051

Plasmid

#242609

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified mU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pJL052

Plasmid

#242610

Purpose

Parental vector for building the dual-sgRNA CRISPRi library. modified hU6-sgRNA library vector

Depositor

Brittany Adamson

Article

Simpson et al bioRxiv [Preprint]. 2023 Aug 20:2023.08.

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pX330_REC8_cterm

Plasmid

#222522

Purpose

Cas9/sgRNA plasmid for targeting REC8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, REC8 sgRNA (REC8 Human, Synthetic)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-GeneTRAP

Plasmid

#196492

Purpose

To KO and genetrap mouse GFAP simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-GeneTRAP

Plasmid

#196493

Purpose

To KO and genetrap mouse VAMP2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GGDestX2-Amp

Plasmid

#157654

Purpose

Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

Depositor

Karmella Haynes

Article

Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

U6, CBh

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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