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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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165,688 results

Showing: 10261 - 10280 of 165688 results

  1. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid
    #154141
    Purpose
    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Article
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Mutation
    R887E
    Promoter
    SFFV
    Available Since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154141-plasmid-map-sequence-id-345617

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  4. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available Since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  5. pHW449

    Plasmid
    #198805
    Purpose
    Direct light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.
    Depositor
    Paul Sternberg
    Article
    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12
    Insert
    15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
    Expression
    Worm
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    198805-plasmid-map-sequence-id-391701

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  7. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-485302

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  14. PX551

    Plasmid
    #60957
    Purpose
    pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
    Depositor
    Feng Zhang
    Article
    Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    HA
    Expression
    Mammalian
    Promoter
    pMecp2
    Available Since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    60957-plasmid-map-sequence-id-181490

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  17. pSin Helper

    Plasmid
    #127694
    Purpose
    Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.
    Depositor
    Justin English, Bryan Roth
    Article
    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306
    Insert
    Sindbis Glycoproteins
    Use
    Synthetic Biology
    Available Since
    July 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 10261 - 10280 of 165688 results