We narrowed to 171,784 results for: Gene

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pEB-Tg

Plasmid

#28220

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Mar;21(3):518-29. doi: 10

Insert

SV40 large T

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSYN-eGFP

Plasmid

#162512

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

hSYN

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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PHY23-phage-ubc-nls-ha-stdMCP-tdTomato

Plasmid

#164044

Purpose

Expression of stdMCP-tdTomato in mammalian cells

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

stdMCP-tdTomato

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM51323

Plasmid

#153224

Purpose

Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ChR2-2A-Halo

Plasmid

#22047

Purpose

Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

Depositor

Edward Boyden

Article

Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

Insert

ChR2-GFP-2A-Halo-YFP

Tags

GFP and YFP

Expression

Mammalian

Mutation

Note that there is a S147R mut in YFP. This mutat…

Promoter

CMV

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Plasmid

#115684

Purpose

NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

Depositor

David Suter

Article

Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

Insert

Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Use

Lentiviral

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-AN

Plasmid

#20950

Depositor

William Kaelin

Article

Neuman et al Mol Cell Biol. 1994 Oct . 14(10):6607-15

Insert

E2F-1 promoter, genomic fragment (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

wt human E2F-1 promoter sequence

Available Since

Aug. 10, 2009

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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human oct4-GFP

Plasmid

#21153

Depositor

Rudolf Jaenisch

Article

Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

Insert

hOCT4-GFP (POU5F1 Human)

Use

Human targeting

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIH-Flag-YAP-S127/381A

Plasmid

#33069

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

S127A, S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
L4440_BioBrick-SCR-sgRNA-B

Plasmid

#177812

Purpose

Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

Scrambled sgRNA targeting sequences

Expression

Bacterial

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PBRM1-pgk-puro

Plasmid

#85746

Purpose

tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

none

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pTF102

Plasmid

#134774

Purpose

A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

Depositor

Kan Wang

Article

Frame et al Plant Physiol. 2002 May;129(1):13-22. do

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBIG1b

Plasmid

#80612

Purpose

pBIG1b - Step1 vector b (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mRuby2

Plasmid

#99114

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pHC01

Plasmid

#106900

Purpose

Ratiometric gas biosensor plasmid for 3-oxo-C12-HSL sensing

Depositor

Jonathan Silberg

Article

Cheng et al ACS Synth Biol. 2018 Jan 24. doi: 10.102

Inserts

LasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein

Expression

Bacterial

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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tdTomato/pTREX-b

Plasmid

#68709

Purpose

Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

Depositor

Roberto Docampo

Article

Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

Insert

tdTomato

Use

Expression of tdtomato red fluorescence protein i…

Available Since

Oct. 8, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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